Characterization and subcellular localization of Alongshan virus proteins

Yinghua Zhao; Ping Wu; Li Liu; Baohua Ma; Mingming Pan; Yuan Huang; Nianyan Du; Hongyan Yu; Liyan Sui; Ze-Dong Wang; Zhijun Hou; Quan Liu; Quan Liu; Quan Liu

doi:10.3389/fmicb.2022.1000322

Frontiers in Microbiology (Sep 2022)

Characterization and subcellular localization of Alongshan virus proteins

Yinghua Zhao,
Ping Wu,
Li Liu,
Baohua Ma,
Mingming Pan,
Yuan Huang,
Nianyan Du,
Hongyan Yu,
Liyan Sui,
Ze-Dong Wang,
Zhijun Hou,
Quan Liu,
Quan Liu,
Quan Liu

Affiliations

Yinghua Zhao: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Ping Wu: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Li Liu: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Baohua Ma: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Mingming Pan: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Yuan Huang: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Nianyan Du: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Hongyan Yu: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Liyan Sui: Department of Infectious Diseases, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China
Ze-Dong Wang: Department of Infectious Diseases, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China
Zhijun Hou: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Quan Liu: College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China
Quan Liu: Department of Infectious Diseases, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China
Quan Liu: School of Life Sciences and Engineering, Foshan University, Foshan, China

DOI: https://doi.org/10.3389/fmicb.2022.1000322
Journal volume & issue: Vol. 13

Abstract

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Alongshan virus (ALSV) in the Jingmenvirus group within the family Flaviviridae is a newly discovered tick-borne virus associated with human disease, whose genome includes four segments and encodes four structural proteins (VP1a, VP1b, VP2, VP3, and VP4) and two non-structural proteins (NSP1 and NSP2). Here, we characterized the subcellular distribution and potential function of ALSV proteins in host cells. We found that viral proteins exhibited diverse subcellular distribution in multiple tissue-deriving cells and induced various morphological changes in the endoplasmic reticulum (ER), and NSP2, VP1b, VP2, and VP4 were all co-localized in the ER. The nuclear transfer and co-localization of VP4 and calnexin (a marker protein of ER), which were independent of their interaction, were unique to HepG2 cells. Expression of NSP1 could significantly reduce mitochondria quantity by inducing mitophagy. These findings would contribute to better understanding of the pathogenesis of emerging segmented flaviviruses.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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