STAR Protocols (Dec 2024)
Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose
- Cyun-Ming Wu,
- Hao-Yun Li,
- Wen-Yi Li,
- Chih-Neng Hsu,
- Wei-Shun Yang,
- Gwo-Tsang Chuang,
- Tony Pan-Hou Che,
- Tung-Yuan Lee,
- Hsiao-Lin Lee,
- Siow-Wey Hee,
- Jiin-Horng Lee,
- Daniel Liao,
- Karen Chia-Wen Liao,
- Ya-Wen Liu,
- Chin-Feng Hsuan,
- Yi-Cheng Chang,
- Shao-Lun Chu
Affiliations
- Cyun-Ming Wu
- Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan
- Hao-Yun Li
- Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan
- Wen-Yi Li
- Division of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan
- Chih-Neng Hsu
- Cardiovascular Center, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan
- Wei-Shun Yang
- Division of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, HsinChu Branch, HsinChu 300, Taiwan
- Gwo-Tsang Chuang
- Division of Nephrology, Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan
- Tony Pan-Hou Che
- PhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan
- Tung-Yuan Lee
- PhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan
- Hsiao-Lin Lee
- Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan
- Siow-Wey Hee
- Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan
- Jiin-Horng Lee
- Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan
- Daniel Liao
- Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan
- Karen Chia-Wen Liao
- Biological Sciences Division, University of Chicago, Chicago, IL 60637, USA
- Ya-Wen Liu
- Institute of Molecular Medicine, National Taiwan University, Taipei 100, Taiwan
- Chin-Feng Hsuan
- Division of Cardiology, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung 824, Taiwan; Division of Cardiology, Department of Internal Medicine, E-Da Dachang Hospital, I-Shou University, Kaohsiung 807, Taiwan; School of Medicine, College of Medicine, I-Shou University, Kaohsiung 824, Taiwan
- Yi-Cheng Chang
- PhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan; Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taiwan 100, Taiwan; Corresponding author
- Shao-Lun Chu
- Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan; Corresponding author
- Journal volume & issue
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Vol. 5,
no. 4
p. 103478
Abstract
Summary: 2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse’s body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
