Quantitative and qualitative analysis for standardization of Euphorbia cuneata Vahl

Abstract

Euphorbia cuneata Vahl very promising plant belongs to Family Euphorbiaceae The present study was carried out on the Euphorbia cuneata Vahl to standardize its components. Qualitative and quantitative phytochemical analysis showed variable phytochemical groups. Examination of Successive Extraction showed that there are different color, constancy, phytochemical groups and yield in each extract, the highest percentage was found in ethanol (10.7 ± 1.01) and the lowest one in ether (1.66 ± 0.31). Analysis of primary and secondary metabolites of Euphorbia cuneata Vahl revealed that the primary metabolites percent (carbohydrate, lipid and protein 6.25 ± 1.11, 5.12 ± 1.40, 7.15 ± 1.31 W/w respectively) were lower than secondary metabolites (flavonoids, phenolic and tannins 11.26 ± 1.02, 9.15 ± 1.21and 5.23 ± 1.29 W/w respectively). The Pharmacopoeia Constants were determined. Amino acids analysis of the arial parts reported the presence of 15 amino acids with different percentage in different types. (Total, free and protein hydrolysate.) Arginine represented the highest concentration (20.86). Keywords: Amino acids, Euphorbia cuneata, Biodiversity, Phytochemical analysis, Pharmacopoeia Constants, Successive Extraction