Analysis on Cloning and Expression of Histone Demethylase Gene OsJMJ719 in Rice

Abstract

【Objective】A novel rice histone demethylase gene OsJMJ719 was cloned. The expression pattern of this gene under abiotic stress was analyzed to provide theoretical basis for exploring the function of OsJMJ719 in response to abiotic stress.【Method】The complete OsJMJ719 gene was obtained by cloning with ZH11 as material. 35S: : OsJMJ719-GFP expression vector integrated with OsJMJ719 and green fluorescent protein was established for subcellular localization. Transient tobacco expression system and rice protoplast transformation method was employed to observe the subcellular localization and real-time PCR was used to analyze the expression profile of OsJMJ719 in different tissues of rice and the expression pattern under abiotic stress treatments.【Result】The coding region of OsJMJ719 gene (LOC_Os02g01940) is 2 994 bp in length, encoding 997 amino acids. The promoter region of OsJMJ719 contains 10 phytohormone response elements and 3 environmental stress regulation related elements. Phylogenetic analysis shows that OsJMJ719 has high homology with JMJ protein in wild Zizania palustris, Aegilops tauschii, Triticum aestivum and Hordeum vulgare. The subcellular localization results reveal that OsJMJ719 protein is localized in the nucleus. Fluorescence quantitative results reveal that OsJMJ719 is highly expressed in seeds, and the expression of this gene is induced by ABA, NaCl and PEG6000, suggesting that it plays an important role in the process of abiotic stress.【Conclusion】The study shows the location of OsJMJ719 in the phylogenetic tree and its homologous species, reveals the localization, structure and characteristics of the protein, and the main external factors responsible for the regulation of this gene. These results provide a fundamental data basis for further research on the function of OsJMJ719 gene.

Keywords

• rice
• osjmj719
• histone demethylase
• gene cloning
• expression analysis