Determination of chloramphenicol in tablets by electrogenerated chemiluminescence

Abstract

Chloramphenicol was determinate by electrogenerated chemiluminescence coupled to FIA using Ru(bpy)3(2+) luminescent complex. Chloramphenicol cause a decrease in luminescence intensity from the ruthenium complex that is proportional to its concentration between 5 x 10-5 and 1 x 10-3 mol L-1. The relative standard deviation was estimated as £ 1.5 % for 1 x 10-4 mol L-1 chloramphenicol solution of 12 successive injections. The chloramphenicol was determined in tablets with success and the soluble components of the matrix did not interfere in the luminescent emission. Results obtained with the luminescent procedure were not statistically different from those obtained by UV-spectrophotometry at the 95 % confidence level.

Keywords

• electrogenerated chemiluminescence
• FIA
• chloramphenicol
• pharmaceutical tablets