Speciation of Candida using chromogenic and cornmeal agar with determination of fluconazole sensitivity

Abstract

Objective and background: Candidiasis is an important cause of fungal infections. This study was carried out to determine the species incidence using chrom agar & cornmeal agar, susceptibility pattern to fluconazole by microbroth dilution methods of yeasts isolated from the clinical samples. Method: Positive samples for candidiasis where collected from 112 patients at Dr B R AMC from April 2010 to April 2011, processed by routine methods, chrom agar media and corn meal agar was used to speciate and antifungal susceptibility done by microbroth dilution method. Results; C.albicans(40), C.tropicalis(30), C.parapsilosis (22), C.krusei (5), C glabrata(15) were obtained. Susceptibility to flucanazole showed MIC C.albicans (0.125-0.5ug/ml), C.tropicalis (0.5-2ug/ml), C.parapsilosis (0.5-4 ug/ml), C.glabrata (16-32ug/ml) C.krusei (32- 64ug/ml), Conclusion: Increasing incidence of non albicans species infection. The MIC of non albicans also showed increased values

Keywords

• Candida
• Chrom agar
• cornmeal agar
• fluconazole