Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro

Abstract

Objective To prepare cisplatin-loaded mPEG-PCL nanoparticles with different proportions of bi-block copolymer mPEG-PCL and PCL, and evaluate their antitumor effect in vitro. Methods mPEG-PCL and HO-PCL were synthesized by ring-opening polymerization method. Nanoparticles (NPs) prepared from mPEG-PCL/PCL hybrids were obtained by mixing PEG-PCL and PCL copolymer in different proportions. Drug-loading nanoparticles were prepared by w/o/w double emulsion method. The in vitro cytotoxicity of cisplatin-loaded nanoparticles on BGC-823 and SGC-7901 cell lines and the toxicity of the blank nanoparticles were evaluated by MTT assay. Results mPEG-PCL and PCL were synthesized with desired molecular weight. In vitro experiments showed the five kinds of nanoparticles had different antitumor activities and consistency in biocompatibility. Conclusion The properties of nanoparticles, such as diameters and drug loading capacities, vary with different ratio of components as well as the antitumor effect. The optimal hybrid proportions should be selected for further study and application.

Keywords

• cisplatin
• nanoparticles
• w/o/w double-emulsion method
• drug-delivery system
• antitumor