Micropropagation of the Cuban hybrid from papaya IBP 42-99

Abstract

This work consisted of developing a methodology to propagate papaya hybrids through in vitro culture. As plant material, lateral buds of plants of the hybrid IBP 42-99 were used. The explants were established following the methodology proposed by Posada et al (1999). For the multiplication stage, the combinations of 6-BAP (0.225, 0.45 and 0.90 mg.l-1) and ANA (0.14 and 0.28 mg.l-1) were studied. In the rooting stage, a comparison was done between the culture media proposed by Drew (1988) and Posada (1995). The culture medium containing MS salts (1962) supplemented with 0.45 mg.l-1 of 6-BAP and 0.14 mg.l-1 of ANA was the most adequate for multiplication, while the best medium for rooting was 5mg.l-1 of AIB with ten days in the culture medium, where 68% of rooted plants was obtained and in the acclimatization phase 71% of plant adaptation was achieved. Keywords: acclimatization, Caricas, in vitro plant culture, rooting