Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine

Abstract

The pharmacokinetic properties of S-amlodipine were studied using racemic amlodipine and single S-enantiomer(SK310) administration to rats. Plasma levels of the drug were determined using chiral liquid chromatography coupled with tandemmass spectrometry following solid phase extraction. The stereospecific analysis of amlodipine was performed on an α-acidglycoprotein (AGP) column using a mobile phase comprising 10 mM ammonium acetate (pH 4.0) and propanol at a flow rate of0.2 mL/min. This method was used to perform a comparative study of the pharmacokinetics of amlodipine and SK310. Theresults revealed that the pharmacokinetic profile of S-amlodipine after the administration of SK310 was comparable to thatfollowing the administration of the racemic mixture.