Preliminary evaluating the effects of umbilical cord-derived mesenchymal stem cells on bile duct ligation induced liver injury mice

Abstract

Objective: The purpose of this research is to evaluate the effective treatment of mesenchymal stem cells due to bile duct obstruction on mouse models with liver damage who underwent Bile Duct Ligation (BDL). Method: Swiss mice underwent bile duct surgery (BDL) to cause cirrhosis. After 07 days of BDL, mice were injected with mesenchymal stem cells isolated from umbilical cord blood-derived (UCB-MSC) at a dose of 5 x 105cell/mice. Treatment efficacy was assessed through mortality, biochemical indices (AST, ALT, Albumin) and histological changes (H&E staining), collagen accumulation in liver tissue (Sirius red staining), and astrocytes activation (immunohistochemistry with α-SMA marker). Result: After 12 days of treatment, compared to the PBS-control group, mice injected with MSC had a high survival rate (100%), reduced liver damage (average liver enzymes of Alb, AST, and ALT, respectively 2,016 Dg/L, 432.9 UI/L, and 417.7 UI/L,), reducing the area of cirrhosis through an average necrotic area of 7.529%, the mean liver collagen accumulation 1.968%, and the positive rate of α-SMA protein is 1.42725%. Conclusion: Initially assess the safety and treatment effectiveness with liver damage caused by bile duct obstruction by Mesenchymal Stem Cells (MSC).

Keywords

• bệnh hẹp đường mật
• chuột tắc mật
• điều trị tắc mật, bệnh lý gan
• liệu pháp tế bào gốc