A CRISPR-Cas9 screen identifies EXO1 as a formaldehyde resistance gene

Yuandi Gao; Laure Guitton-Sert; Julien Dessapt; Yan Coulombe; Amélie Rodrigue; Larissa Milano; Andréanne Blondeau; Nicolai Balle Larsen; Julien P. Duxin; Samer Hussein; Amélie Fradet-Turcotte; Jean-Yves Masson

doi:10.1038/s41467-023-35802-y

Nature Communications (Jan 2023)

A CRISPR-Cas9 screen identifies EXO1 as a formaldehyde resistance gene

Yuandi Gao,
Laure Guitton-Sert,
Julien Dessapt,
Yan Coulombe,
Amélie Rodrigue,
Larissa Milano,
Andréanne Blondeau,
Nicolai Balle Larsen,
Julien P. Duxin,
Samer Hussein,
Amélie Fradet-Turcotte,
Jean-Yves Masson

Affiliations

Yuandi Gao: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Laure Guitton-Sert: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Julien Dessapt: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Yan Coulombe: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Amélie Rodrigue: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Larissa Milano: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Andréanne Blondeau: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Nicolai Balle Larsen: Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
Julien P. Duxin: Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
Samer Hussein: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Amélie Fradet-Turcotte: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center
Jean-Yves Masson: CHU de Québec Research Center, Oncology Division, Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center

DOI: https://doi.org/10.1038/s41467-023-35802-y
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 20

Abstract

Read online

Formaldehyde can trigger formation of interstrand crosslinks (ICLs) or DNA-protein crosslinks (DPCs) leading to genome instability. Here the authors show that EXO1 limits replication stress and DNA damage to counteract formaldehyde-induced genome instability.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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