Construction of characterization parameters of algal photosynthetic inhibition method for detection of comprehensive toxicity in water

Mengyuan Gu; Gaofang Yin; Tingting Gan; Nanjing Zhao; Peilong Qi; Min Chen; Zhichao Ding; Renqing Jia; Jinjing Liu; Mingjun Ma; Ruifang Yang; Li Fang; Wenqing Liu

Ecological Indicators (Mar 2022)

Construction of characterization parameters of algal photosynthetic inhibition method for detection of comprehensive toxicity in water

Mengyuan Gu,
Gaofang Yin,
Tingting Gan,
Nanjing Zhao,
Peilong Qi,
Min Chen,
Zhichao Ding,
Renqing Jia,
Jinjing Liu,
Mingjun Ma,
Ruifang Yang,
Li Fang,
Wenqing Liu

Affiliations

Mengyuan Gu: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Gaofang Yin: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China; Corresponding authors.
Tingting Gan: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China; Corresponding authors.
Nanjing Zhao: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Peilong Qi: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Min Chen: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Zhichao Ding: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Renqing Jia: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Jinjing Liu: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Mingjun Ma: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Ruifang Yang: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Li Fang: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
Wenqing Liu: Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China; Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China

Journal volume & issue: Vol. 136
p. 108651

Abstract

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Pollutants in water seriously threaten the health of human beings and will lead to potential ecological risks to the aquatic environment. Researchers use many chemical, physiological and morphological methods to detect the comprehensive toxicity in water. Among them, the photosynthetic inhibition method is considered a powerful method to detect the comprehensive toxicity in water, takes the chlorophyll fluorescence in the body as a natural probe, and determines the toxicity effect of pollutants by measuring the variation of photosynthetic fluorescence parameters. It has the advantages of fast and convenient measurement and real-time online detection. The widely used photosynthetic fluorescence parameters are the maximal quantum yield of photosystem II (Fv/Fm) and performance index PIABS. There are the problems that the sensitivity is not high enough, and the substances that can be detected are limited when researchers use Fv/Fm and PIABS as indicators to detect the comprehensive toxicity in water. So, in this paper, we proposed a construction method of Performance Index of Comprehensive Toxicity Effect (PICTE) using multi-parameter fusion based on the chlorophyll a fluorescence rise kinetics curve (OJIP) of algae. The fluorescence information of point J and point I in the curve was integrated. The area surrounded by the curve and the time corresponding to point J and point I were weighted for data items to construct PICTE. On this basis, Chlorella vulgaris was used as the tested algae species to compare the performance differences of Fv/Fm, PICTE, PIABS in the detection of toxic substances under the stress of five common water pollutants: Diuron (DCMU, 3-(3,4-dichlorophenol)-1,1-dimethylurea), Dibromothymoquinone (DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone), Methyl viologen (MV, 1,1ꞌ-dimethyl-4,4ꞌ-bipyridinium-dichloride), Malathion, Carbofuran. The performance differences of Fv/Fm, PICTE, PIABS in detecting toxic substances, including lowest detection limit, highest response concentration, stability, and reproducibility, were studied. The results show that: in terms of toxicity detection, the constructed PICTE was sensitive to all five toxic substances and could detect more kinds of pollutants than Fv/Fm. The lowest detection limit of PICTE for five toxic substances compared with PIABS was reduced by 74.43%, 77.01%, 67.30%, 60.69%, and 50.81%, respectively. At the same time, it has a stronger ability to detect toxic substances with high concentration. The stability and reproducibility of PICTE for detecting the toxicity of DCMU, DBMIB, Malathion, and Carbofuran were all excellent, and all of the relative standard deviation (RSD) was < 5%. This research provides an important basis for constructing a highly sensitive and high-precision detection method for comprehensive toxicity in water.

Published in Ecological Indicators

ISSN: 1470-160X (Print); 1872-7034 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Ecology
Website: https://www.sciencedirect.com/journal/ecological-indicators

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