Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

Ricardo D. Righetto; Nikhil Biyani; Julia Kowal; Mohamed Chami; Henning Stahlberg

doi:10.1038/s41467-019-09661-5

Nature Communications (Apr 2019)

Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

Ricardo D. Righetto,
Nikhil Biyani,
Julia Kowal,
Mohamed Chami,
Henning Stahlberg

Affiliations

Ricardo D. Righetto: Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel
Nikhil Biyani: Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel
Julia Kowal: Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel
Mohamed Chami: Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel
Henning Stahlberg: Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel

DOI: https://doi.org/10.1038/s41467-019-09661-5
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to determine the structure at 4 Å resolution.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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