Basophil activation test in the diagnosis of allergic reactions to local anesthetics

Abstract

Objective: to evaluate the detection of in vitro hypersensitivity to local anesthesia drugs by fl ow cytometry.Materials and methods: in the test of activation of basophils, the determination of the hypersensitivity reaction to local anesthetics of groups I, II (154 preparations) in 106 patients and T-lymphocytes of type 2 immune response (CD3 + CD294 +) was carried out by fl ow cytometry using the Allergenicity kit test system. In the history of the examined patients, there was a certain food and/ or drug intolerance.Results: Th e levels of sensitization to diff erent groups of local anesthetics were compared in the basophil activation test. Th e highest levels of hypersensitivity were observed to local anesthetics containing vasoconstrictors, stabilizers. In patients with hypersensitivity to drugs for local anesthesia, an increase in the number of T-lymphocytes of type 2 of the immune response was observed.Conclusions: based on the identifi ed hypersensitivity in the basophil activation test to local anesthesia drugs, the safest anesthetics were established. Th e diagnostic signifi cance of determining the level of T-lymphocytes of type 2 of the immune response in drug hypersensitivity is shown.

Keywords

• basophil activation test
• hypersensitivity
• local anesthetics
• flow cytometry