Biosynthesis and Characterisation of Ellagic Acid Coupled Silver NanoparticlesAn In-vitro Study

Abstract

Introduction: Nanomedicine dominated the world of therapeutics and diagnostics. Horizons are extending daily to identify a piece of the puzzle that helps us produce ecofriendly and cost-effective nanoparticles. In this, authors tried to synthesise a novel Silver Nanoparticle Coated with Ellagic Acid (EA-AgNPs) obtained from pomegranate using a simple method and explore its characterisation precisely. Aim: To biosynthesise and assess the characterisation of Ellagic Acid coupled Silver Nanoparticles. Materials and Methods: The present in-vitro study assessed the portrayal of EA-AgNPs using various analytical techniques. Firstly, to analyse: the size of the EA-AgNPs using Ultraviolet (UV)- Visible Spectrometer; Secondly to determine the hydrodynamic size and its dispersity using Dynamic Light Scattering (DLS) and Zeta potential; thirdly to quantify the average size of EA-AgNPs by using Scanning Electron microscope (SEM); and lastly to identify the functional groups by using Fourier Transform Infrared Spectroscopy (FTIR) method. Descriptive statistics were used to analyse the results. Results: Brownish colour change and shift of peak wavelength from 430-423 nm using UV-Visible Spectrometer analysis confirmed the formation and stability of EA-AgNPs. The DLS analysis revealed that EA-AgNPs were in nanosize (129.7 nm) with less aggregated polydispersity index (0.483). The Zeta potential confirmed that this newly synthesised nanoparticle was negatively charged -0.268 mv. The SEM determination confirmed the formation of spherical-shaped nanoparticles with sizes ranging from 84.34-98.80 nm. The FTIR revealed EA-AgNPs exhibited different functional groups, which help to prevent particle aggregation. Conclusion: In this work, the novel EA-AgNPs exhibited the apt characterisation needed for an effective and cost-efficient nanoparticle that could be effectively tapped in various fields of nanodentistry.

Keywords

• analytical technique
• capping agents
• green chemistry
• nanomedicine
• reducing agents