The determination of topiramate in plasma rabbit blood by HPLC with specific derivatization

Abstract

Тhe paper of Shohin I.E., Ramenskaya G.V., Medvedev Yu.V., Yarushok T.A., Sheveleva M.A.1 is devoted to development and validation of HPLC method determination of topiramate in rabbit plasma with precolumn derivatization. Topiramate was extracted from plasma by dichloromethane. Derivatization was performed by dansyl chloride in borate buffer. HPLC analysis was performed on C18 column (3 μm, 4,6 x 75 mm). Acetonitrile – 25 mM phosphate buffer pH 2.50 (30:70) at 1.0 mL/min was used as mobile phase. The standard curve was linear over the range 0.95 μg/ml to 47.32 μg/ml of topiramate in plasma. Within-run precision was 1.55 % to 2.12 % and between-run precision was 1.67 % to 8.09 % determined on spiked samples. The accuracy of the method was 1.14 % to 19.62 % (within-run) and 1.74 % to 19.25 (between-run). The lower limit of quantification was 0.95 μg/ ml. The method was applied to preclinical pharmacokinetics study of topiramate drug products in rabbits.

Keywords

• topiramate
• plasma
• rabbits
• hplc
• derivatization