Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose

Maria Bawn; Fabiana Subrizi; Gary J. Lye; Tom D. Sheppard; Helen C. Hailes; John M. Ward

Data in Brief (Aug 2018)

Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose

Maria Bawn,
Fabiana Subrizi,
Gary J. Lye,
Tom D. Sheppard,
Helen C. Hailes,
John M. Ward

Affiliations

Maria Bawn: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Bernard Katz Building, London WC1E 6BT, UK
Fabiana Subrizi: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK
Gary J. Lye: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Bernard Katz Building, London WC1E 6BT, UK
Tom D. Sheppard: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK
Helen C. Hailes: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK
John M. Ward: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Bernard Katz Building, London WC1E 6BT, UK; Corresponding author.

Journal volume & issue: Vol. 19
pp. 1341 – 1354

Abstract

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The dataset presented in this article is related to the research article entitled “One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose” (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigate new thermostable transketolase (TK) activities with l-arabinose. Transaminase (TAm) sequences from an in-house library of thermophilic strains were analyzed to compare homologies to characterized TAms with desired activity. DNA and amino acid sequences are presented for all the enzymes investigated. Calibration curves for products of the TK and TAm reactions are also presented along with chromatographic analysis of the various one-pot reactions.

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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