Association of Adiponectin Gene rs2241766 Polymorphism with non-Alcoholic Fatty Liver Disease in an Iranian Population

Abstract

Background and Aim: Adiponectin is the most abundant peptide extracted from adipose tissue, which plays an important role in development of obesity. The purpose of this study was to evaluate the association of single-nucleotide polymorphism rs2241766 (+45T>G) with biochemical and anthropometric parameters in patients with non-alcoholic fatty liver. Methods: The present case-control study was performed on 80 patients with non-alcoholic fatty liver and 80 healthy peoples as control (77 men and 83 women). Triglyceride, total cholesterol, HDL, LDL levels and adiponectin levels were measured by standard methods using Pars Azmoon kit and ELISA kit respectively. Genotyping was also performed by PCR-RFLP method. Data were analyzed using independent t-test, Mann-Whitney, Chi-square and Kolmogorov-Smirnov tests. Results: Frequency of TG genotype was higher in patients (52.4%) and frequency of TT genotype in healthy people (52.7%), but regression analysis showed that rs2241766 polymorphism genotypes did not play a role in fatty liver disease (p<0.05). The present study showed that none of the variables in the carriers of TT, TG, and GG genotypes was significantly different in the healthy group (p<0.05), however, in the patients, the level of cholesterol in the genotypes was significantly different (p=0.02). Conclusion: The results indicated that although the frequency of GT genotype and G allele in non-alcoholic fatty liver were more common. No significant relationship was seen between rs2241766 polymorphism in adiponectin genemm, non-alcoholic fatty liver disease and adiponectin levels.

Keywords

• Non-alcoholic Fatty Liver Disease
• Adiponectin
• Polymorphism
• Lipid metabolism.