Protective effects of hexane fraction of Costus afer leaves against sodium arsenite-induced hepatotoxicity and nephrotoxicity in male albino wistar rats

Abstract

Arsenite is a toxic metallic pollutant known to cause hepatotoxic and nephrotoxic injuries. Costus afer Ker Gawl. is an indigenous medicinal plant used as therapy for numerous tissue disorders. Thus, this study investigated the protective potential of C. afer hexane leaf fraction (CALHF) on sodium arsenite-induced hepatic and renal injuries in albino rats. Twenty-five male albino rats were randomly distributed into five groups of five rats each. Group 1: rats administered orally with 0.5 ml of 0.9% saline; Group 2: untreated rats induced with 5 mg/kg body weight (b.w.) sodium arsenite (i.p.); Group 3: rats induced with sodium arsenite and treated with 10 mg/kg b.w. silymarin (hepatoprotective drug); Group 4 and 5: rats induced with sodium arsenite and treated with 100 and 200 mg/kg b.w. CALHF, respectively. CALHF was orally administered daily, while sodium arsenite was administered every 48 hours for 14 days. Thereafter, rats were sacrificed, blood was collected to estimate hepatic and nephrotic functions. Hepatic and renal function tests showed that 100 and 200 mg/kg CALHF and 10 mg/kg silymarin treated animals had significantly reduced (P < 0.05) plasma alanine aminotransferase, aspartate aminotransferase, creatinine and urea levels, when compared with those of untreated animals. C. afer hexane leaf fraction exhibited hepatoprotective and nephroprotective effects against sodium arsenite induced toxicity in rats.

Keywords

• costus afer
• hepatotoxicity
• nephrotoxicity
• sodium arsenite
• liver enzymes