Glioma Associated Stem Cells (GASCs) Isolation and Culture

Evgenia Bourkoula; Damiano Mangoni; Federica Caponnetto; Tamara Ius; Miran Skrap; Antonio Beltrami; Daniela Cesselli

doi:10.21769/BioProtoc.1457

Bio-Protocol (May 2015)

Glioma Associated Stem Cells (GASCs) Isolation and Culture

Evgenia Bourkoula,
Damiano Mangoni,
Federica Caponnetto,
Tamara Ius,
Miran Skrap,
Antonio Beltrami,
Daniela Cesselli

Affiliations

Evgenia Bourkoula: Department of Medical and Biological Sciences, University of Udine, Udine, Italy
Damiano Mangoni: Department of Medical and Biological Sciences, University of Udine, Udine, Italy
Federica Caponnetto: Department of Medical and Biological Sciences, University of Udine, Udine, Italy
Tamara Ius: Department of Neurosurgery, University Hospital of Udine, Udine, Italy
Miran Skrap: Department of Neurosurgery, University Hospital of Udine, Udine, Italy
Antonio Beltrami: Department of Medical and Biological Sciences, University of Udine, Udine, Italy
Daniela Cesselli: Department of Medical and Biological Sciences, University of Udine, Udine, Italy

DOI: https://doi.org/10.21769/BioProtoc.1457
Journal volume & issue: Vol. 5, no. 9

Abstract

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Glioma Associated Stem Cells (GASCs) represent a population of non-tumorigenic multipotent stem cells hosted in the microenvironment of human gliomas. In vitro, these cells are able, through the release of exosomes, to increase the biological aggressiveness of glioma-initiating cells. The clinical importance of this finding is supported by the strong prognostic value associated with the GASCs surface immunophenotype thus suggesting that this patient-based approach can provide a groundbreaking method to predict prognosis and to exploit novel strategies that target the tumor stroma (Bourkoula et al., 2014).

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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