Activity of Bacteriophage Preparation in Relation to Antibiotic-Resistant El Tor Strain of Vibrio Cholerae

Abstract

The spread of Vibrio cholerae strains with multiple antibiotic resistances makes it necessary to search for alternative antibiotics that are effective against the cholera pathogen. The aim of the study was to investigate the lytic activity of cholera phages from the collection of the bacteriophage laboratory in vitro and in vivo with respect to antibiotic-resistant genovariants of V.cholerae El Tor. As a result of the in vitro study, it was possible to identify the promising phages for phagotherapy of experimental cholera. Comparative evaluation of the effectiveness of the phage mixture in relation to the antibiotic-resistant El Tor 19243 strain of V.cholerae in vivo on a model of a generalized infection in white mice showed high effectiveness of its preventive and therapeutic use (more than 70% of surviving animals).

Keywords

• cholera bacteriophages
• poly-antibiotic-resistant strains
• phage prophylaxis
• antibacterial drugs