Single-cell RNA sequencing of human nail unit defines RSPO4 onychofibroblasts and SPINK6 nail epithelium

Hyun Je Kim; Joon Ho Shim; Ji-Hye Park; Hyun Tae Shin; Jong Sup Shim; Kee-Taek Jang; Woong-Yang Park; Kyung-Hoon Lee; Eun Ji Kwon; Hyung-Suk Jang; Hanseul Yang; Jong Hee Lee; Jun-Mo Yang; Dongyoun Lee

doi:10.1038/s42003-021-02223-w

Communications Biology (Jun 2021)

Single-cell RNA sequencing of human nail unit defines RSPO4 onychofibroblasts and SPINK6 nail epithelium

Hyun Je Kim,
Joon Ho Shim,
Ji-Hye Park,
Hyun Tae Shin,
Jong Sup Shim,
Kee-Taek Jang,
Woong-Yang Park,
Kyung-Hoon Lee,
Eun Ji Kwon,
Hyung-Suk Jang,
Hanseul Yang,
Jong Hee Lee,
Jun-Mo Yang,
Dongyoun Lee

Affiliations

Hyun Je Kim: Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine
Joon Ho Shim: Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine
Ji-Hye Park: Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine
Hyun Tae Shin: Department of Dermatology, Inha University School of Medicine
Jong Sup Shim: Department of Orthopedic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine
Kee-Taek Jang: Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine
Woong-Yang Park: Samsung Genomic Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine
Kyung-Hoon Lee: Department of Anatomy, Sungkyunkwan University School of Medicine
Eun Ji Kwon: Morristown Pathology Associates
Hyung-Suk Jang: Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine
Hanseul Yang: Department of Biological Science, Korea Advanced Institute of Science and Technology
Jong Hee Lee: Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine
Jun-Mo Yang: Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine
Dongyoun Lee: Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine

DOI: https://doi.org/10.1038/s42003-021-02223-w
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 13

Abstract

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Kim et al. conducted single-cell RNA sequencing to determine the transcriptome profiles of human nail units using polydactyly specimens to demonstrate mesenchymal and epithelial cell populations, characterized by RSPO4 and SPINK6 respectively. RSPO4 + onychofibroblasts localized with LGR6 + nail matrix, leading to WNT/ β-catenin activation and suggesting a role for onychofibroblasts in onychomatricoma pathogenesis.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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