Genetic diversity of bonytongue fish Heterotis niloticus (Cuvier, 1829) in Cameroon, Central Africa

Jeanne Wikondi; Felix Meutchieye; Christopher Adenyo; Richard Osei-Amponsah; Boniface Baboreka Kayang; Minette Eyango Tabi Tomedi

Scientific African (Nov 2023)

Genetic diversity of bonytongue fish Heterotis niloticus (Cuvier, 1829) in Cameroon, Central Africa

Jeanne Wikondi,
Felix Meutchieye,
Christopher Adenyo,
Richard Osei-Amponsah,
Boniface Baboreka Kayang,
Minette Eyango Tabi Tomedi

Affiliations

Jeanne Wikondi: Department of Genetics and Biostatistics, School of Veterinary Medicine and Sciences, University of Ngaoundere, PO Box: 454, Ngaoundere, Cameroon; Department of Animal Science, Biotechnology and Bioinformatics Research Unit, University of Dschang, FASA, PO Box. 188, Dschang, Cameroon; Corresponding author at: Department of Genetics and Biostatistics, School of Veterinary Medicine and Sciences, University of Ngaoundere, PO Box: 454, Ngaoundere, Cameroon.
Felix Meutchieye: Department of Animal Science, Biotechnology and Bioinformatics Research Unit, University of Dschang, FASA, PO Box. 188, Dschang, Cameroon
Christopher Adenyo: Livestock and Poultry Research Centre, School of Agriculture, University of Ghana, P. O. LG38, Legon, Accra, Ghana
Richard Osei-Amponsah: Department of Animal Sciences, School of Agriculture, University of Ghana, PO Box. LG226, Legon, Accra, Ghana
Boniface Baboreka Kayang: Department of Animal Sciences, School of Agriculture, University of Ghana, PO Box. LG226, Legon, Accra, Ghana
Minette Eyango Tabi Tomedi: Institute of Fisheries and Aquatic Sciences of the University of Douala, Yabassi PO Box. 7236, Douala-Bassa, Cameroon

Journal volume & issue: Vol. 22
p. e01975

Abstract

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The African bonytongue (Heterotis niloticus) fish is the only species of the Osteoglossidae family in Africa. The characterization of this fish was carried out from January to June 2020 in Cameroon where this fish is distributed in native zones. This study aimed at contributing to the genetic characterization of Heterotis niloticus population in Cameroon. A total of 96 Heterotis niloticus were sampled from five regions namely Centre, South, Littoral, North and Far North. Caudal fins where randomly collected for genomic DNA (gDNA) extraction. Following standard procedures for simple sequence repeat markers available for this species, seven microsatellites were used for genotyping through Polymerase Chain Reaction (PCR) and electrophoresis. Genetic parameters were scored using the GenAlex 6.503 software and the population structure of Heterotis niloticus was assessed under STRUCTURE Version 2.3.4 software. The results showed that the local population of Heterotis niloticus in North-Cameroon has a higher number of allele (Na=6.00) and effective number of allele (Ne=3.66) while the highest Observed Heterozygosity (Ho) was observed for the population of Heterotis niloticus in the Far-North. The highest genetic distance is observed between the population of Far North and Centre, Far-North and South, Far-North and North. In general, similarity within the sub-population of Heterotis niloticus is higher than 65%. Genetically, the population of Heterotis niloticus in Cameroon was grouped into three genetic sub-populations based on microsatellites appraisal. Further investigations are suggested for analysis concerning productive traits for domestication and aquaculture purposes.

Published in Scientific African

ISSN: 2468-2276 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: https://www.journals.elsevier.com/scientific-african

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