Frontiers in Immunology (Jun 2022)
High-Dimensional Immune Profiling by Mass Cytometry Revealed the Circulating Immune Cell Landscape in Patients With Intracranial Aneurysm
- Peicong Ge,
- Peicong Ge,
- Peicong Ge,
- Peicong Ge,
- Peicong Ge,
- Chenglong Liu,
- Chenglong Liu,
- Chenglong Liu,
- Chenglong Liu,
- Chenglong Liu,
- Liujia Chan,
- Yuheng Pang,
- Hao Li,
- Hao Li,
- Hao Li,
- Hao Li,
- Hao Li,
- Qian Zhang,
- Qian Zhang,
- Qian Zhang,
- Qian Zhang,
- Qian Zhang,
- Xun Ye,
- Xun Ye,
- Xun Ye,
- Xun Ye,
- Xun Ye,
- Jia Wang,
- Jia Wang,
- Jia Wang,
- Jia Wang,
- Jia Wang,
- Rong Wang,
- Rong Wang,
- Rong Wang,
- Rong Wang,
- Rong Wang,
- Yan Zhang,
- Yan Zhang,
- Yan Zhang,
- Yan Zhang,
- Yan Zhang,
- Wenjing Wang,
- Dong Zhang,
- Dong Zhang,
- Dong Zhang,
- Dong Zhang,
- Dong Zhang,
- Dong Zhang,
- Jizong Zhao,
- Jizong Zhao,
- Jizong Zhao,
- Jizong Zhao,
- Jizong Zhao,
- Jizong Zhao
Affiliations
- Peicong Ge
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Peicong Ge
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Peicong Ge
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Peicong Ge
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Peicong Ge
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Chenglong Liu
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Chenglong Liu
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Chenglong Liu
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Chenglong Liu
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Chenglong Liu
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Liujia Chan
- Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing, China
- Yuheng Pang
- Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing, China
- Hao Li
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Hao Li
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Hao Li
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Hao Li
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Hao Li
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Qian Zhang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Qian Zhang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Qian Zhang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Qian Zhang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Qian Zhang
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Xun Ye
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Xun Ye
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Xun Ye
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Xun Ye
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Xun Ye
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Jia Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jia Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jia Wang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Jia Wang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Jia Wang
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Rong Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Rong Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Rong Wang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Rong Wang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Rong Wang
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Yan Zhang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Yan Zhang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Yan Zhang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Yan Zhang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Yan Zhang
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Wenjing Wang
- Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing, China
- Dong Zhang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Dong Zhang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Dong Zhang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Dong Zhang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Dong Zhang
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Dong Zhang
- Department of Neurosurgery, Beijing Hospital, Beijing, China
- Jizong Zhao
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jizong Zhao
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jizong Zhao
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Jizong Zhao
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China
- Jizong Zhao
- Beijing Translational Engineering Center for 3D Printer in Clinical Neuroscience, Beijing, China
- Jizong Zhao
- Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
- DOI
- https://doi.org/10.3389/fimmu.2022.922000
- Journal volume & issue
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Vol. 13
Abstract
BackgroundIncreasing evidence supports a critical role of chronic inflammation in intracranial aneurysm (IA). Understanding how the immunological alterations in IA provides opportunities for targeted treatment. However, there is a lack of comprehensive and detailed characterization of the changes in circulating immune cells in IA.ObjectiveTo perform a comprehensive and detailed characterization of the changes in circulating immune cells in patients with IA.MethodsPeripheral blood mononuclear cell samples from IA patients (n = 26) and age-and sex-matched healthy controls (HCs, n = 20) were analyzed using high dimensional mass cytometry, and the frequency and phenotype of immune cell subtypes were assessed.ResultsWe identified 28 cell clusters and found that the immune signature of IA consists of cluster changes. IA patients exhibited dysfunction of immunity, with dysregulation of CD4+ T-cell clusters, increased B cells and monocytes, and decreased CD8+ T cells, DNT cells, and DPT cells. Moreover, compared with findings in HC, IA was associated with enhanced lymphocyte and monocyte immune activation, with a higher expression of HLA-DR, CXCR3, and CX3CR1. In addition, the expression of TLR4, p-STAT3, and the exhaustion marker PD1 was increased in T cells, B cells, and NK cells in IA patients.ConclusionsOur data provide an overview of the circulating immune cell landscape of IA patients, and reveal that the dysfunction of circulating immunity may play a potential role in the development of IA.
Keywords
