PLoS ONE (Jan 2020)

Antimicrobial activity of Asteraceae species against bacterial pathogens isolated from postmenopausal women.

  • Marcela Oliveira Chiavari-Frederico,
  • Lidiane Nunes Barbosa,
  • Isabela Carvalho Dos Santos,
  • Gustavo Ratti da Silva,
  • Alanna Fernandes de Castro,
  • Wanessa de Campos Bortolucci,
  • Lorena Neris Barboza,
  • Caio Franco de Araújo Almeida Campos,
  • José Eduardo Gonçalves,
  • Jacqueline Vergutz Menetrier,
  • Ezilda Jacomassi,
  • Zilda Cristiani Gazim,
  • Samantha Wietzikoski,
  • Francislaine Aparecida Dos Reis Lívero,
  • Evellyn Claudia Wietzikoski Lovato

DOI
https://doi.org/10.1371/journal.pone.0227023
Journal volume & issue
Vol. 15, no. 1
p. e0227023

Abstract

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PURPOSE:Investigation of the antibacterial action of aqueous extracts of Bidens sulphurea, Bidens pilosa, and Tanacetum vulgare, species of Asteraceae family that are popularly used for the treatment of genito-urinary infection. METHODS:The minimum inhibitory concentration (MIC) and minimal bacterial concentration (MBC) of the extracts against standard strains of Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC25922), and Pseudomonas aeruginosa (ATCC27853) and against bacteria that were isolated from cultures of vaginal secretions and urine from menopausal women with a diagnosis of recurrent urinary tract infections (rUTI) were determined by broth microdilution. RESULTS:The MIC values of the three extracts against Gram-positive and Gram-negative standard bacterial strains ranged from 7.81 to 125.00 mg ml-1, and the MBC values ranged from 7.81 to 500.00 mg ml-1. However, B. sulphurea was more efficient. In the urine samples, the three extracts inhibited the growth of coagulase-negative Staphylococcus spp., and the B. pilosa was the most active extract against E. coli compared with the other ones. For the vaginal secretion samples, no significant differences in the inhibition of coagulase-positive Staphylococcus spp. and P. mirabilis were found among the extracts. T. vulgare and B. sulphurea were more effective in inhibiting coagulase-negative Staphylococcus spp. compared with B. pilosa. E. coli was more susceptible to the B. sulphurea extract compared with the B. pilosa and T. vulgare extracts. CONCLUSION:The present results suggested the potential medicinal use of Asteraceae species, especially B. sulphurea, as therapeutic agents against rUTI-related bacteria.