Broadband CARS high-throughput single-cell imaging

Abstract

Broadband Coherent anti-Stokes Raman Scattering (BCARS) enables the whole vibrational spectrum of cytologically prepared samples to be obtained using a hyperspectral raster scan approach. This technique has the potential to enable high-throughput automated detection of cell abnormalities. Images are distorted by the non-resonant background which requires a treatment for proper analysis. Using statistical denoising and phase retrieval returns Raman spectra similar to that of a spontaneous Raman measurement. Here, we present our work using this method for single-cell imaging of PEO1 ovarian adenocarcinoma cells prepared with the ThinPrep processor which enables label-free Raman cytology.