Genetic Variations and mRNA Expression of Goat <i>DNAH1</i> and Their Associations with Litter Size

Zhen Wang; Ruolan Wang; Chuanying Pan; Hong Chen; Lei Qu; Lian Wu; Zhengang Guo; Haijing Zhu; Xianyong Lan

doi:10.3390/cells11081371

Cells (Apr 2022)

Genetic Variations and mRNA Expression of Goat <i>DNAH1</i> and Their Associations with Litter Size

Zhen Wang,
Ruolan Wang,
Chuanying Pan,
Hong Chen,
Lei Qu,
Lian Wu,
Zhengang Guo,
Haijing Zhu,
Xianyong Lan

Affiliations

Zhen Wang: Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Ruolan Wang: Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Chuanying Pan: Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Hong Chen: Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Lei Qu: Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, China
Lian Wu: Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Zhengang Guo: Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China
Haijing Zhu: Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, China
Xianyong Lan: Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China

DOI: https://doi.org/10.3390/cells11081371
Journal volume & issue: Vol. 11, no. 8
p. 1371

Abstract

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Dynein Axonemal Heavy Chain 1 (DNAH1) encodes proteins which provide structural support for the physiological function and motor structure of spermatozoa (hereafter referred to as sperm) and ova. This study found that three single nucleotide polymorphisms (SNPs), the 27-bp insertion/deletion (InDel) mutations and three exonic copy number variations (CNVs) within DNAH1 were significantly associated with litter size of Shaanbei white cashmere goats (n = 1101). Goats with the wildtypes of these three SNPs had higher litter sizes than other carriers (p p = 0.000022). The gain genotype had the largest litter sizes compared with the loss or medium carriers for the three CNV mutations (p 1-M2-M3 and AA-TT-CC-II-G1-G2-M3 combination genotypes had larger litter sizes compared with the other genotypes. This study also showed the DNAH1 expression in mothers of multiple kids was higher than mothers of single kids. These three SNPs, the 27-bp InDel and three CNVs in DNAH1 could be used as molecular markers for the selection of goat reproductive traits.

Published in Cells

ISSN: 2073-4409 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Cytology
Website: https://www.mdpi.com/journal/cells

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