Journal of Enzyme Inhibition and Medicinal Chemistry (Jan 2020)

Imidazothiazole-based potent inhibitors of V600E-B-RAF kinase with promising anti-melanoma activity: biological and computational studies

  • Hanan S. Anbar,
  • Mohammed I. El-Gamal,
  • Hamadeh Tarazi,
  • Bong S. Lee,
  • Hong R. Jeon,
  • Dow Kwon,
  • Chang-Hyun Oh

DOI
https://doi.org/10.1080/14756366.2020.1819260
Journal volume & issue
Vol. 35, no. 1
pp. 1712 – 1726

Abstract

Read online

A series of imidazothiazole derivatives possessing potential activity against melanoma cells were investigated for molecular mechanism of action. The target compounds were tested against V600E-B-RAF and RAF1 kinases. Compound 1zb is the most potent against both kinases with IC50 values 0.978 and 8.2 nM, respectively. It showed relative selectivity against V600E mutant B-RAF kinase. Compound 1zb was also tested against four melanoma cell lines and exerted superior potency (IC50 0.18-0.59 µM) compared to the reference standard drug, sorafenib (IC50 1.95-5.45 µM). Compound 1zb demonstrated also prominent selectivity towards melanoma cells than normal skin cells. It was further tested in whole-cell kinase assay and showed in-cell V600E-B-RAF kinase inhibition with IC50 of 0.19 µM. Compound 1zb induces apoptosis not necrosis in the most sensitive melanoma cell line, UACC-62. Furthermore, molecular dynamic and 3D-QSAR studies were done to investigate the binding mode and understand the pharmacophoric features of this series of compounds.

Keywords