Germination of somatic embryos of <i>Psidium guajava</i> L. cv. Cuban Red Dwarf EEA 18-40 in temporary immersion systems

Abstract

Somatic embryo germination of Psidium guajava L. cv. Cuban Red Dwarf EEA 18-40 in temporary immersion systems (TIS), in which somatic embryos were cultured in the heart-torpedo stage in MS mediun at mayor half strength salt and suplemented with: 0.25 mg.l-1 of 6-bencilaminopurine (6-BAP), 10 mg.l-1 of Biobras-6 (analogous of brasinoesteriode) and 20 g.l-1 of sucrose. As control was used solid cultivation medium (2.5 g.l-1 Gellan gum, Spectrum®) of same composition to the one used in the TIS. The variables germination percentage and fresh weight were evaluated statistically. After ten weeks of cultivation the largest values in germination percentage (91.04%) and fresh weight (1.22 g) were obtained in the TIS, being statistically different to those obtained in solid medium (9.79% and 1.03 g, respectively). Key words: in vitro plant, guayaba, regeneration, RITA®,somatic embryogenesis