PLoS ONE (Jan 2024)

Search for carbapenem-resistant bacteria and carbapenem resistance genes along swine food chains in Central Italy.

  • Cristiana Garofalo,
  • Cristiana Cesaro,
  • Vesna Milanović,
  • Luca Belleggia,
  • Tullia Matricardi,
  • Andrea Osimani,
  • Lucia Aquilanti,
  • Federica Cardinali,
  • Giorgia Rampanti,
  • Serena Simoni,
  • Carla Vignaroli,
  • Andrea Brenciani,
  • Marina Pasquini,
  • Maria Federica Trombetta

DOI
https://doi.org/10.1371/journal.pone.0296098
Journal volume & issue
Vol. 19, no. 1
p. e0296098

Abstract

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The presence of carbapenem-resistant bacteria and carbapenem resistance genes (CRGs) in livestock is increasing. To evaluate the presence of carbapenemase-producing Enterobacteriaceae (CPE) and the main CRGs along swine food chains of the Marche Region (Central Italy), samples of faeces, feed, and animal-food derived products were collected from seven small/medium, medium, and large-scale pig farms. A total of 191 samples were analysed using a culture-dependent method, with the aim of isolating CPE. Isolates were analysed for their resistance to carbapenems using a modified Hodge test and the microdilution method for the minimum inhibitory concentration (MIC) determination. Moreover, the extraction of microbial DNA from each sample was performed to directly detect selected CRGs via qPCR. Among the 164 presumptive resistant isolates, only one strain from a liver sample, identified as Aeromonas veronii, had an ertapenem MIC of 256 μg/mL and carried a carbapenemase- (cphA) and a β-lactamase- (blaOXA-12) encoding genes. A low incidence of CRGs was found; only nine and four faecal samples tested positive for blaNDM-1 and blaOXA-48, respectively. Overall, the importance of monitoring CPE and CRGs in livestock and their food chains should be stressed to control all potential non-human CPE and CRGs reservoirs and to determine safety levels for human health.