Animals (Jan 2024)

The Effects of Composite Alkali-Stored Spent <i>Hypsizygus marmoreus</i> Substrate on Carcass Quality, Rumen Fermentation, and Rumen Microbial Diversity in Goats

  • Shuiling Qiu,
  • Keyao Li,
  • Xiangbo He,
  • Mingming Gu,
  • Xinghui Jiang,
  • Jianing Lu,
  • Zhiyi Ma,
  • Xuewu Liang,
  • Qianfu Gan

DOI
https://doi.org/10.3390/ani14010166
Journal volume & issue
Vol. 14, no. 1
p. 166

Abstract

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The objective of this study was to investigate the effects of composite alkali-stored spent Hypsizygus marmoreus substrate (SHMS) on carcass quality, rumen fermentation, and rumen microbial diversity in goats. Twenty-four 6-month-old Chuanzhong black goats with similar body weights (20 ± 5 kg) were selected and randomly divided into four groups (n = 6 per group) and received four treatments: 0% (control group, CG); 20% (low-addition group, LG); 30% (moderate-addition group, MG); and 40% (high-addition group, HG) of SHMS-replaced silage corn and oat hay. The experiment lasted for 74 days (including a 14 d adaptation period and a 60 d treatment period). The results of this study showed that MG and HG significantly improved the marble score of goat meat (p p p p > 0.05). The cooking loss in MG was higher than that in CG (p p p > 0.05). The rumen pH of MG significantly decreased (p 3-N) increased by 44.63% and 54.50%, respectively. The addition of the SHMS altered both the alpha and beta diversities of the rumen microbiota and significant differences in the composition and structure of the four microbial communities. The dominant bacterial phylum in each group were Firmicutes and Bacteroidetes, with Prevotella 1 as the dominant bacterial genus. Correlation analysis revealed that rumen bacteria are closely related to the animal carcass quality and rumen fermentation. In the PICRUSt prediction, 21 significantly different pathways were found, and the correlation network showed a positive correlation between the Prevotella 1 and 7 metabolic pathways, while the C5-branched dibasic acid metabolism was positively correlated with nine bacteria. In summary, feeding goats with an SHMS diet can improve the carcass quality, promote rumen fermentation, and alter the microbial structure. The research results can provide a scientific reference for the utilization of SHMS as feed in the goat industry.

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