A Taste Bud Organoid-Based Microelectrode Array Biosensor for Taste Sensing

Shuge Liu; Ping Zhu; Yulan Tian; Yating Chen; Yage Liu; Miaomiao Wang; Wei Chen; Liping Du; Chunsheng Wu

doi:10.3390/chemosensors10060208

Chemosensors (Jun 2022)

A Taste Bud Organoid-Based Microelectrode Array Biosensor for Taste Sensing

Shuge Liu,
Ping Zhu,
Yulan Tian,
Yating Chen,
Yage Liu,
Miaomiao Wang,
Wei Chen,
Liping Du,
Chunsheng Wu

Affiliations

Shuge Liu: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Ping Zhu: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Yulan Tian: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Yating Chen: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Yage Liu: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Miaomiao Wang: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Wei Chen: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Liping Du: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
Chunsheng Wu: Department of Biophysics, Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China

DOI: https://doi.org/10.3390/chemosensors10060208
Journal volume & issue: Vol. 10, no. 6
p. 208

Abstract

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The biological taste system has the unique ability to detect taste substances. Biomaterials originating from a biological taste system have been recognized as ideal candidates to serve as sensitive elements in the development of taste-based biosensors. In this study, we developed a taste bud organoid-based biosensor for the research of taste sensation. Taste bud organoids prepared from newborn mice were cultured and loaded onto the surface of a 64-channel microelectrode array (MEA) chip to explore the electrophysiological changes upon taste; an MEA chip was used to simultaneously record multiple-neuron firing activities from taste bud organoids under different taste stimuli, which helped to reveal the role of taste buds in taste sensing. The obtained results show that taste cells separated from the taste epithelium grew well into spherical structures under 3D culture conditions. These structures were composed of multiple cells with obvious budding structures. Moreover, the multicellular spheres were seeded on a 64-channel microelectrode array and processed with different taste stimuli. It was indicated that the MEA chip could efficiently monitor the electrophysiological signals from taste bud organoids in response to various taste stimuli. This biosensor provides a new method for the study of taste sensations and taste bud functions.

Published in Chemosensors

ISSN: 2227-9040 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: http://www.mdpi.com/journal/chemosensors

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