Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

Sho Yoshimatsu; Tsukika Sato; Masafumi Yamamoto; Erika Sasaki; Mayutaka Nakajima; Mari Nakamura; Seiji Shiozawa; Toshiaki Noce; Hideyuki Okano

Stem Cell Research (Apr 2020)

Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

Sho Yoshimatsu,
Tsukika Sato,
Masafumi Yamamoto,
Erika Sasaki,
Mayutaka Nakajima,
Mari Nakamura,
Seiji Shiozawa,
Toshiaki Noce,
Hideyuki Okano

Affiliations

Sho Yoshimatsu: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Proteolytic Neuroscience, RIKEN Center for Brain Science, Saitama, Japan
Tsukika Sato: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Masafumi Yamamoto: ICLAS Monitoring Center, Central Institute for Experimental Animals, Kanagawa, Japan
Erika Sasaki: Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kanagawa, Japan
Mayutaka Nakajima: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Mari Nakamura: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Seiji Shiozawa: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Toshiaki Noce: Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan
Hideyuki Okano: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Corresponding author at: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.

Journal volume & issue: Vol. 44

Abstract

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BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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