Antimicrobial and Immunomodulating Activities of Two Endemic <em>Nepeta</em> Species and Their Major Iridoids Isolated from Natural Sources

Neda Aničić; Uroš Gašić; Feng Lu; Ana Ćirić; Marija Ivanov; Bojan Jevtić; Milena Dimitrijević; Boban Anđelković; Marijana Skorić; Jasmina Nestorović Živković; Yingle Mao; Jia Liu; Chunping Tang; Marina Soković; Yang Ye; Danijela Mišić

doi:10.3390/ph14050414

Pharmaceuticals (Apr 2021)

Antimicrobial and Immunomodulating Activities of Two Endemic <em>Nepeta</em> Species and Their Major Iridoids Isolated from Natural Sources

Neda Aničić,
Uroš Gašić,
Feng Lu,
Ana Ćirić,
Marija Ivanov,
Bojan Jevtić,
Milena Dimitrijević,
Boban Anđelković,
Marijana Skorić,
Jasmina Nestorović Živković,
Yingle Mao,
Jia Liu,
Chunping Tang,
Marina Soković,
Yang Ye,
Danijela Mišić

Affiliations

Neda Aničić: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Uroš Gašić: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Feng Lu: State Key Laboratory of Drug Research and Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu-Chong-Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China
Ana Ćirić: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Marija Ivanov: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Bojan Jevtić: Department of Immunology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Milena Dimitrijević: Center of Excellence for Green Technologies, Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia
Boban Anđelković: Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11000 Belgrade, Serbia
Marijana Skorić: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Jasmina Nestorović Živković: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Yingle Mao: State Key Laboratory of Drug Research and Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu-Chong-Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China
Jia Liu: State Key Laboratory of Drug Research and Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu-Chong-Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China
Chunping Tang: State Key Laboratory of Drug Research and Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu-Chong-Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China
Marina Soković: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Yang Ye: State Key Laboratory of Drug Research and Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu-Chong-Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China
Danijela Mišić: Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia

DOI: https://doi.org/10.3390/ph14050414
Journal volume & issue: Vol. 14, no. 5
p. 414

Abstract

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Two Balkan Peninsula endemics, Nepeta rtanjensis and N. argolica subsp. argolica, both characterized by specialized metabolite profiles predominated by iridoids and phenolics, are differentiated according to the stereochemistry of major iridoid aglycone nepetalactone (NL). For the first time, the present study provides a comparative analysis of antimicrobial and immunomodulating activities of the two Nepeta species and their major iridoids isolated from natural sources—cis,trans-NL, trans,cis-NL, and 1,5,9-epideoxyloganic acid (1,5,9-eDLA), as well as of phenolic acid rosmarinic acid (RA). Methanol extracts and pure iridoids displayed excellent antimicrobial activity against eight strains of bacteria and seven strains of fungi. They were especially potent against food-borne pathogens such as L. monocytogenes, E. coli, S. aureus, Penicillium sp., and Aspergillus sp. Targeted iridoids were efficient agents in preventing biofilm formation of resistant P. aeruginosa strain, and they displayed additive antimicrobial interaction. Iridoids are, to a great extent, responsible for the prominent antimicrobial activities of the two Nepeta species, although are probably minor contributors to the moderate immunomodulatory effects. The analyzed iridoids and RA, individually or in mixtures, have the potential to be used in the pharmaceutical industry as potent antimicrobials, and in the food industry to increase the shelf life and safety of food products.

Published in Pharmaceuticals

ISSN: 1424-8247 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceuticals/

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