Haematologica (Aug 2008)

Molecular analysis of immunoglobulin variable genes in human immunodeficiency virus-related non-Hodgkin’s lymphoma reveals implications for disease pathogenesis and histogenesis

  • Daniela Capello,
  • Maurizio Martini,
  • Annunziata Gloghini,
  • Michaela Cerri,
  • Silvia Rasi,
  • Clara Deambrogi,
  • Davide Rossi,
  • Michele Spina,
  • Umberto Tirelli,
  • Luigi Maria Larocca,
  • Antonino Carbone,
  • Gianluca Gaidano

DOI
https://doi.org/10.3324/haematol.12705
Journal volume & issue
Vol. 93, no. 8

Abstract

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Background Human immunodeficiency virus (HIV)-related non-Hodgkin’s lymphomas (HIV-NHL) are heterogeneous and associated with distinct molecular pathways. Analysis of immunoglobulin variable genes (IGV) may provide insights into the pathogenesis and histogenesis of HIV-NHL.Design and Methods IGV rearrangements were amplified from genomic DNA by polymerase chain reaction and directly sequenced in 87 cases of HIV-NHL (17 Burkitt/Burkitt-like lymphomas, 38 diffuse large B-cell lymphomas, and 32 primary central nervous system lymphomas).Results A skewed IGHV repertoire in specific HIV-NHL clinico-pathological categories was observed. Systemic HIV-diffuse large B-cell lymphomas displayed underrepresentation of the IGHV3 family (11/38, 28.9%; p=0.0047) and, in particular, of the IGHV3–23 gene (0/38; p