Memorias do Instituto Oswaldo Cruz (Feb 1945)
Estudos sobre a dosagem microbiologica das vitaminas do complexo B - II. Niacina Microbiological assay for niacine
Abstract
The microbiological assay method of Snell and Wright for niacine was studied and some modifications of the basal medium were proposed. A maximal growth of the "Lactobacillus arabinosus" was obtained by the addition to the basal medium of 25 mg % asparagine and increasing the percentages of glucose and sodium acetate. Liver and yeast extracts were assayed satisfactory and the niacine added was recovered quantitatively.