Memorias do Instituto Oswaldo Cruz (Feb 1945)

Estudos sobre a dosagem microbiologica das vitaminas do complexo B - II. Niacina Microbiological assay for niacine

  • Gilberto G. Villela,
  • Alberto B. Hargreaves

DOI
https://doi.org/10.1590/S0074-02761945000100013
Journal volume & issue
Vol. 42, no. 1
pp. 183 – 190

Abstract

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The microbiological assay method of Snell and Wright for niacine was studied and some modifications of the basal medium were proposed. A maximal growth of the "Lactobacillus arabinosus" was obtained by the addition to the basal medium of 25 mg % asparagine and increasing the percentages of glucose and sodium acetate. Liver and yeast extracts were assayed satisfactory and the niacine added was recovered quantitatively.