Effect of dental antiseptic agents on the viability of human periodontal ligament cells

Abstract

Objectives: We aimed to study whether or not various dental antiseptic agents affect the viability and proliferation of human periodontal ligament cells (PDLCs). Materials and methods: Human PDLCs were isolated from a total of 10 surgically extracted impacted third molars and were.cultured in-vitro. The cells were exposed to commonly used dental antiseptics, including chlorhexidine, cetylpyridinium chloride, triclosan, povidone-iodine and sodium bicarbonate for ultra-short-term (10, 20, 30 sec), short-term (10, 20, 30 min) and long-term (24, 48 h) at various concentrations. Cell morphology was observed with light microscopy. Cell viability was studied with impedimetric real-time xCELLigence and resazurin-based alamarBlue® assays. We used one-way ANOVA with Tukey’s and Bonferroni test (p chlorhexidine > triclosan > povidone-iodine > sodium bicarbonate. Conclusions: Our findings suggest that the tested antiseptic agents were cytotoxic to human PDLCs at lower than practically applied concentrations in dental interventions.

Keywords

• Periodontal ligament cells
• Dental antiseptic compounds
• Cell viability
• Impedimetry