Remineralization of Demineralized Bone Matrixes with Preserved Fibrillary Structure as a Promising Approach to Obtain Highly Effective Osteoplastic Materials

Abstract

The development of highly effective osteoplastic materials capable of providing bone tissue regeneration still remains an urgent and unresolved problem. In the presented work, an approach is proposed for the creation of biomimetic materials by the deposition of amorphous calcium phosphates on the surface of a xenogenic bone demineralized matrix under physiological conditions. Adsorption spectroscopy and scanning electron microscopy showed the efficiency of deposition of amorphous calcium phosphates on the trabeculae surface. The additional inclusion of the calcium-binding protein albumin was found to increase the efficiency of CPC adsorption on the trabeculae surface during DBM remineralization in vitro. In the model of heterotopic implantation for 7 weeks the osteoinductive properties of the obtained material were demonstrated, expressed in intrabecular mineralization of bone trabeculae, neovascularization and pronounced synthetic activity of osteoblasts (synthesis and structurization of neocollagen directly on the implanted material). The data obtained in the course of this work will be used to create new highly effective osteoplastic materials.