Differential ion mobility mass spectrometry in immunopeptidomics identifies neoantigens carrying colorectal cancer driver mutations

Yuriko Minegishi; Kazuma Kiyotani; Kensaku Nemoto; Yoshikage Inoue; Yoshimi Haga; Risa Fujii; Naomi Saichi; Satoshi Nagayama; Koji Ueda

doi:10.1038/s42003-022-03807-w

Communications Biology (Aug 2022)

Differential ion mobility mass spectrometry in immunopeptidomics identifies neoantigens carrying colorectal cancer driver mutations

Yuriko Minegishi,
Kazuma Kiyotani,
Kensaku Nemoto,
Yoshikage Inoue,
Yoshimi Haga,
Risa Fujii,
Naomi Saichi,
Satoshi Nagayama,
Koji Ueda

Affiliations

Yuriko Minegishi: Cancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research
Kazuma Kiyotani: Project for Immunogenomics, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research
Kensaku Nemoto: Project for Immunogenomics, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research
Yoshikage Inoue: Department of Surgery, Kyoto University
Yoshimi Haga: Cancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research
Risa Fujii: Cancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research
Naomi Saichi: Cancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research
Satoshi Nagayama: Development of Gastroenterological Surgery, Cancer Institute Hospital of Japanese Foundation for Cancer Research
Koji Ueda: Cancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research

DOI: https://doi.org/10.1038/s42003-022-03807-w
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 13

Abstract

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An optimized method for mass spectrometry based immunopeptidomics analysis using high-field asymmetric waveform ion mobility spectrometry is presented and identifies 44,815 HLA-bound peptides (HLAp) from colorectal cancer samples.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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