Sequence analysis of the ribosomal DNA ITS2 region in two Trichogramma species (Hymenoptera: Trichogrammatidae)

Abstract

Two egg parasitoid wasps, Trichogramma euproctidis (Girault) and Trichogramma brassicae (Bezdenko) (Hymenoptera: Trichogrammatidae) were identified in the study. The taxonomy of these wasps is problematic because of their small size and lack of distinguishable morphological characters. The DNA sequence variation from the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA (rDNA) was analyzed from these two Trichogramma species. This technique provides quick, simple and reliable molecular identification of Trichogramma species.

Keywords

• Trichogramma euproctidis
• Trichogramma brassicae
• molecular identification
• rDNA ITS2 region