Selection of the optimal concentration of indole-3-butyric acid (IBA) in the nutrient medium for effective micropropagation of Populus × berolinensis

Abstract

The study describes the features of the effective micropropagation of Populus berolinensis using indolyl-3-butyric acid (IBA). Internode sections without axillary buds are the most frequently used type of explant for Agrobacterium-mediated genetic transformation of woody plants. The application of growth regulators, such as indolyl-3-butyric acid (IBA), allows to increase the length of the internodes and thereby solve the problem of the availability of a sufficient amount of material for genetic transformation. Our results showed that 0.15 mg L-1 of IBA in the nutrient medium is the most suitable concentration for the effective micropropagation of Berlin poplar. Shoots rooted or regenerated from axillary buds in case of replanting of the rooted stump at medium supplemented with IBA in mentioned concentration, have expanded internodes in comparison with the control group of plants and may be successfully used as a good source of internode sections in Agrobacterium-mediated plant transformation.