The Loop-Mediated Isothermal Amplification for the Rapid Detection of Porphyromonas Gingivalis

Abstract

Periodontitis is a highly prevalent oral disease occurring across the globe, resulting from an interaction of many predisposing factors. Our study aimed to determine some chosen periopathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, Tannarella forsythia) using three different methods: loop-mediated isothermal amplification (LAMP), polymerase chain reaction (PCR) and real-time PCR (qPCR), and to compare their sensitivity and specificity. The study included 62 patients with diagnosed periodontitis. Gingival crevicular fluid was collected from all patients to take samples of bacterial DNA for the further laboratory molecular analyses. In comparison to the gold standard (qPCR), the best diagnostic quality parameters were achieved for LAMP using the TE buffer for the P. gingivalis determination. Therefore, the LAMP is an analytical technique that could be used to quickly assess the presence of periopathogens in an outpatient setting.

Keywords

• loop-mediated isothermal amplification
• periodontal disease
• periodontal pathogen
• periodontal bacteria
• periodontal diagnostics
• <i>Porhyromonas gingivalis</i>