Column chromatography USED in purification of genotoxic impurity sample of imatinib mesylate substance

Abstract

The article describes a simple method for purifying N-(5-amino-2-methylphenyl)-4-(3-pyridyl)-2-pyrimidineamine for further use as a standard for quantitative determination of genotoxic impurities substance imatinib mesylate. Experimentally proved choice of sorbent used for purification of N-(5-amino-2-methylphenyl)-4-(3-pyridyl)-2-pyrimidineamine. The advantage of column chromatography on neutral alumina prior to crystallization methods presented in the literature. Comparative chromatographic and spectral characteristics of N-(5-amino-2-methylphenyl)-4-(3-pyridyl)-2-pyrimidineamine before and after purification. NMR spectroscopy evaluated quantitative content of the target substance in the purified sample.

Keywords

• n-(5-амино-2-метилфенил)-4-(3-пиридил)-2-пиримидинамин
• иматиниба мезилат
• генотоксичная примесь
• колоночная хроматография
• нейтральный оксид алюминия
• вэжх
• спектроскопия ямр
• n-(5-amino-2-methylphenyl)-4-(3-pyridyl)-2-pyrimidinamine
• imatinib mesylate
• genotoxic impurity
• column chromatography
• neutral alumina
• hplc
• nmr spectroscopy