Sains Medika (Jun 2022)

Effect of Eucheuma cottonii extract on interleukin-1 level and caspase-3 expression in borax-induced rat’s hepatocyte

  • Ahmad Muhyi,
  • Joko Wahyu Wibowo,
  • Sri Priyantini

DOI
https://doi.org/10.30659/sainsmed.v13i1.25075
Journal volume & issue
Vol. 13, no. 1
pp. 30 – 34

Abstract

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Borax is a hepatotoxic substance and induces oxidative stress in hepatocytes. It may trigger the expression of pro-inflammatory cytokines like Interleukin-1 and apoptotic signals like Caspase-3. Extract of the seaweed Eucheuma cottonii contains carotenoids and flavonoids which suppress oxidative stress in hepatocyte. This study was conducted to determine the effect of E. cottonii extract on borax-induced Interleukin-1 and Caspase-3 expression in rat hepatocyte cells. This research employs a posttest-control group design. Twenty Wistar male rats were separated into four groups: control group (K1) received no intervention, K2 received Na-CMC 0.5% for 17 days and Borax 40 mg/200 g on the fourth day every hour for 14 days, P1 and P2groups were administered E. cottonii extracts at doses of 600 mg/200 g and 1200 mg/200 g for 17 days, respectively, followed byborax induction at a dose of 40 mg/200 g on the fourth day with a one-hour interval for 14 days. Interleukin-1 (IL-1) levels were measured with ELISA, while caspase-3 expression was measured with PCR. For the statistical analysis, Kruskal Wallis and Mann Whitney were used. The level of IL-1 in P2 was significantly lower than in K2 (p<0.05). Caspase-3 expression was significantly lower in P1 and P2 than in K2 (p<0.05). IL-1 level and caspase-3 expression of hepatocytes are affected by administration of E. cottonii extract. The extract has the ability to prevent inflammation and hepatocyte apoptosis. <img 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CvqB/5GcA7E6mtoCUorL5dlcuAIVJdWYtYECVT68VV48URCOHUDzYxgc9q4ZvkSxmR7SNMbMPcVIevraLakk/FREE2EaZyZF33vHQ62JIVC8bFg9erV1NfXU1JS0uM+eXl5pKWlsXbt2kEc2eCyZMkSHn/88Yvq911aWspDDz3Eli1bBnBkHRkUB3BFRUXCz3n06FHlAO4DSUlJpGakYXPa2FdTys8qfsd79ScAgdPiQEOgIYBoyc2INIhIA7vFzrFwOccayzklyvn7cV9i9qhc0tLTwJQJdABHX4YKl3V1ChatyePhkyt5Yf1y2l6Tyti4Yinr6Px5N2dek8fDm3swIm1dS96qQvK7ewk7+yo/XrMFZ8F3+NtPZPbr6i4GTYPgCSdNvxpDhh4k1NzCtC/pzPuvUdhTJMGjPmR9GM0wEGY0Ow4BmiYgxYr48njkymzC9x/A+PMprFKS/WQ9p660EJza3wV87L4Xd93S7T28jBnecqL1b7srfTeoXq5IKdGExuvePTRqLZjNYTSbhsPlwPC2cAY7P/ScZ4a9Ep/PQlJKFVm+X1FhXx2NeE/oC1XPOkX+hZ9vHRl8o1Kw9jTVfg2Xy4XL5cJm64/hLW7g6GgEkrGACEOaHUpp9eb8jaORIPdij/oUo6+yOr6RFXeto7ibua7te0aeAX5E6NRF61PiGKpAJYj2kY1mk0YdipHdO2j60XcwK88hUlLB54sadeM9ZiMRhKYhnEmEXn8Jf3Iy1plXtcpo4sSJ+P1+gsHgIF/J5Y+SVbs1f8nQ6GpfME0TwwyD1GhqOkdl5fv84Y8bkDKCMwluvdVNfX2Y85Uhrpzh5Gy5yalTYcKhAMdP7KFk/z6unDEFKSVWaxq6LhBCT0zpxh7nqvxLmle6fwcbIQgRdSpJkynT83CWN9Fc/jqHTpzjaIqH3FmzmHrVVdiSXbTU1uGXJmGXk9raGk6/8zbVx04Q8bZQe66CtLRk8q5bzL69RbT4mkC39nNww2+e6kL1Hl7ctIcGtzu6xrvyJu6YPTABnMNVp3q1IQAXY4/o7zi+d/5f2PjfdzGh07YjL3+fl49E1+ButxuXazYL7pzLgFguhrVOtWPrWvI2jYvJpIi1ecXkd5ZhN8/Kgs6BYj09T7vR07LnVrD0yWJsNhsul4uCH7zBffPiWw+y5dubORSTkTuuU60/Y5h81XTSbIARIBwRaHr/quOYphntuyw1Kis/4siRt/nls//VJ5069MHb7Ni1kytnTME0TVJTx6PrAk2zJGae+tNKnF9a382GW1iz9xUenNX/rxgpRF9xJa9XjuLdc04aqqsQJjQ0hGmqCuKWoOla9D3YkDiS7Ny28tNkTc0iZMBrd75P2rmdjHH48FoyyDgbxkhx4x0/oy1bIUHEg/O66Ek/iOpNbp9sU7s23MnP3umoOzOXPMjdN4xKzGAUCsUl01en7uOPP87q1asvWyfw448/zkMPPdRnJ3Dc+fv4448PwujaGLQewIrBR9M1XEkuUl3JFH70Ki81vsUB70k8FjcBGSJsRvAH6slPuZpPuK8mnSSKfO/xZssBPDhxaHaswsLBxpP83txKsCnMfTmfIeQM0GRtIhLuf2nNojUdnb/xRXQr+Z2PyGX5+hdgxVLWPndjr86OxTcUwOZCireuZnGnxUrZmTIAiivKoPNrW2UNR5nGXdMGz/kLICMS40+p5Pit1ARr8Fwlmf9YKi17Gzj09Q+wV9Xjlj6chHCEI1jRsWDFREM4rYixDrT/uQbrU7PRD57BOF2Du0En/bdnOPdvV4CegIVgT86LYcKJbT/lHftn+dr8nAH7jhEhp0s0+A0aNXvZ8ofzTClYwpWpg/OVpjTRhEZZoJy3m/YhIxKpgZCQ7HFxriXMVYT4ZupZwlKgaQbhFp1R+u+pd34Gv2N2zGGV4PJKPQS/LM1bN0yDK3ycLz1Mg3MyUyamkxCzjYxm/gqhobczMOhCwyK0aD/f2OcWTcciNCyajtmLE7jVadxfg8Wi1ZSUrO76ecyhy4Ib+2b4m7qc1Q9uZ+mTxRQ9srjTy24ZGzdFA5KGrc52w8jQqWhwxNIVDB/j+iCRmZmJxWIhAshjR2j84bcxa2sQySmYzc1YZ8zEPu8m9KnTogamY6UEd28nfHA/9tuW4FqxEmnKaOs/wGKxkJWVxenTp4f60i47lKyKKN4M+Q/28Xk6RAihY7Pa2P/eU7zyyru8/fb7fPWrydTVBqiuifDv3y4nM3MyE8ZfwckyJ8c/OsSZM8dZ9a3RVFVHOFsu+fo3HuCvP3cr99xzJ+6kfKQ0EhgHM5Brv5FT+QAJwogwOnsKN8y6jrojL3Pzpz6FluTg1NHjHN6xi4Dfz6eWLWP25+4g4G3i/556hg+KtuHOSGfUhAlkTpuKzyIwKivJy/8M3oY6DpfuIRRKUFDFcJ6nRl/PnQ9k8O6z7xCccxs3T08esK8arjrVmw0BgOM72F48MM+szrYQ903d7VVFA5/iq9+aR0bsk4oDL3LwDTvX3Dqj9bOEMRJ0iqh9J39BQVQmW4spzB9HQfsdjm9kxaZxvFBS0ia3rWvJW5XXzbPtws/TNifW+ui6fvtPuHPNndj//UW+eh3AHJZ8fw5LuhzZzJkDJVQ6UnDZSXC5Ig1dc7Dlpf/gxRffYuvW9/jqV5OprfH3SafKK+DzX1hOwV1L+Ju/uZvcnDsSXKBs8Jy9Bw4cAGDu3LkX3Hfjxo0sX758oIfUihBwOJDO23UZNHlr0DWobYpQ3xBBhgw0PVoJSxcC3aIhkTRVNZExMYNAbQPeuhAtJ5ykZtbi0n2E3JL00+8RzMgm5EzkM7uI4s355OcXU7i7iNWLLtYO2J+1wzZ+cusPeHPZ93lx4/y2jw9v4beqra5CMWS89dZb/OpXv2p9xvbGnDlz+NrXvsZDDz3EfffdNwijGxqmT5/eZydwe+fvxWQMJ4JBdQC3F/hTTz1FXl4eeXl5F32ep556KpHDumxJSU7BbrcjNcGfmrZzKHASKU0CZhgnFtI0F3+V+ymuSZrONPtkkoSFM1X1vOktRtNdGBiEzDCmNDkaOMX/CZ2v6EvQHQ5SPMnU1tX1b4DHN1K4OZ+VmzovJKIRt6zJ4+GT3R2Yy/K7Cli3qpCie3uJGluUTwGFMWdvh1hNduyMvVht7mqMLzn8JvBp0ga5Wq1eZmPMHgd+Sy34Gpnw2RSsbjj1t/tw1tbgcfpw+pqwhwPY091YIjo0BQAH4tlPg8eK/Lt30EqXIb6YheWxI0ibnfQ3qqj70hiCV1x+ZXOGAiWnkUvEjLClcTtSFxCWCMAAkiw6DakeNugHSdGaCEiBLkCaAqs9wjjveo7b1oEYnJ6lix8poeSGteStWsraCX15YVrM6l7KriSUmnLOej1MnpQg5y/RMsJCCEzTpCHY3LZBaOBv6NDHtz7YTNjfQI0ZaetH1g3JdndiotV7oGzndoop4NGLcNDn3ltAwZMPU/hcAYvbH7e1kHXFBTy6fsjNvhfN8Nepxax+rIDCVdvZcXw5uYPsYB/3mX9lw2cG9zsBnE4naWlpmIAl4Kfhf36Meb4SLS0ZGQiQdPfXcH39frQkV6vdUdx8K467vkLwtS3YP3k7Wlo6Imb1i2tSWloalZWVIyqzdLijZDUysFp0yk4dorR0L1u37qKxsZycSVBVHaS+PomGeg+f+cwN1NV6qatrRNNsTL9iDnPnXM3uPXtwu5tJSw2TnmZw5MPD/PwZya231jN+/AzSUydiSmOoL7EDix8pYZBWFYnHCGNzJXPN9Gv4mwWL+X3JYfI/eSsLr5iOBCqB/9u1k1/+4HscP19Oc3UNH504zmcf/SHJuROwCEGS08nBrW9zeMurZE4Yz1Uz51JTV8nZs6UDMOChnaeGimGtUzEbQk8Oj+gaMJ+VCwZq3dZmC/ne+e62ZzLvjo6B6tlzr6fpjcNUVuSQke1M7HBGiE6VVRSTe0NUJmVnymBSfkcH/dTlrO+cgLpoNY8uK+ThTRspWHRx1ZcKnyym4LH1bXakhf/I6g/e5Gf/9zJ/dd0djO3pUG8VdQ2QOWcMifT/CiSlR4vZv/9tXnxxG3V1p5mYI6msCvRZp1JTQqSlRjh44D1+8mOTz3++ienT8xibdUXC4zUHmoKCApqamnj11Vd7dQLfd999/PrXv0ZKyVe+8pVBGVsEjb2+VJojGkJKapsiBIIG/oCBUwgCfgMZCIDpw8BHyGlBs2hYbBY0i45uETQ3hKk66WSyu4ZQvSQr3Upz9VFqJ16XuIFuLaYwfyEv3JXL0lXdBTFfGrn3rqfk3t73Ofn87/kTn+P798/vuGHmEu5OwBgUCsWl8eyzz/LLX/6yz/t/4xvf4Oabb+61TPTlQF+cwEPp/IVBdgDff//9rf+PO4Dbf9ZXlAO4b6SkpGC1WgjIEC97d+Mzgzh1O0EZwWU6GSdS+eecLzLRNg47LgC2thwEM4wmBBFTggCHZuN0qIJqo5Y6M0iS1UKKx9NvB3DZzu0U5y9kdbsX3faLgaLeDl6UTwEP9xyZGz0b4/KhcOcOyu7NbVvQx6J2C5YVULi5jIrjQOsYzlJ9DrhpGrMv9cIuEXeJRmbAR52lHoujiZTJKQSPNZLkPYXT04zD14hzTjqO//g82qwsAOT+auSqbXC0ApGTgqioQB5vQJvqREofUoaxNxskv3uOauVYTAhKTiOPiBnBolnY13yEUvMMBE1MTATREt3SqnP16FwW6KchIhBBjWg5YoNwwEKyaw8p/m00uj4FZgS0QZg6FxWwMr+QdZcUbTtw+Pw+IA2rPXHnFEIQMQySrDb+dPsa7Lol2ltWaETMCHMy2swxT938DzQEW7BoWhf/r4x2yiRoRCh4fQ3+SBg9gSWw2ogFES0ruMiX4MUUPJjP0ifbBy+1Zf8OHylfmBGpUx8jkpOT0XWdiATRXIRt7K8Jj5qNUd2C65678fzDt5BSYsZKCUcMogEVrmTsS7+MBCKRaHa2EKDFfnRdJzU1lcrKyqG+xMsGJauRgcNh5ejRYt54cyOvv1bHjCt15i/wUF4RoqU5DV/zBD592xfZuettzp3bRiRiZ8qU2Vw1Zy7/srqUWTMNZs8KkZPj4MMPT/LO22W4PfXcqH+RrNGTCYaGlwN4pJOZMY7RE3LITsnADBscra5g31vbOL7vPZyZo0iZdSX3PPEjHl/6FTKm5PKFNf/B4TffoeaXH9FSWc2k667GOTmHYChMSEom5Exi0snJVNacGepLu2wY3jq1mPxlUNhNoHjrGjB/JTcOgLO+z7aQQWb461Q0U3Hc0uhvZRXF5Gd3U8GnG3Kz86HbpINe2FpMIQU82skONX/uHfzspwc4VH4HY8d1f2hzdTUNZDMx7SK/8wIIYVJc/Aa//8P/JECnTrBt63Fs9ko0TTBu7DRkYlOVB5zCwkJuu+02br/99h6dwHHn7/LlywfN+QvgFU5OmykEWpoIhCBiQigMkYgkEDC5bXE2X7prHhPHOak5WcXJd0ujJaEBhEBKgSCCr8aOt0pnTOo5Ag0O3PXHaciejWFxJGCUsXfUBS+Qu6isD7bXxHKyYi9c/Yku5e8VCsXQcvDgwYvavy+ZwpcLvTmBh9r5C9E2eYrLlGSHjXJZzcs1u7DrDqzCimlKJCY2knDKVD5qqmNbzfu8WPkWhjQImaHYoiKakSUQmKZEF1asmoPiut1Uh6tIcrj7Obroy1N+X8tndiH2Yra7t1ejXG5ckA/F29lxvN3HZ8oppoD8R/IpoJjtO8vatp3ez8EP4RMzru56uoYP2fHii7zxxhvs2rWLj6oT1Qc5Str7XnStjCR5GpfjJJYkP7KxiST7aezmKZxXW3C+dC/6vDHIV/chX30PcctYtLcL4OfFmPduQgYboMmHTBJIGYCIDyn8JO0pT+hYu2Xr2tas/vjP2q0XOqiMjSva71vE2rw8VjxX1u1+8x/d1v1pDm9hzZo1vHIIKN3K5s2befnlD6iJbQ7Wnubs2bPU19cTCkrkRGkAACAASURBVIX6cZGXgZza3fPWnxUb6XzHuxCXb2zfsudWkJe3tqtxYuta8vJWsKnbE1bx7q+/zxPPvU0FFex76XlefPFFSk61xLY3UvPRR1RWVtLc3NzdCS4aKSW60GmO+HitZTdG2GzNHJWAJgRWu4VvpN5KddIKpMOC0ERsF4kpQYZNxvp+gR5pAqFfsP9sYog9vzYXt97jsudWRO//8Y2syMsjL28FG49DR72JyXdNN8/GmGw2tnsebnt0Prfeeit33nkny5cv57f7ex9VUno6Hqqor01cVpmUMjrnILglezbzx8xgwZgrmZc1nYVjZ5Fsa+uNPTs9h4VjZzIvawbzx3T8WTDmSuaPmcEt2bPRhDZwctpayLrifFYuvXiXbe69BdGskvgzLpb9WxDLCO5Nr26+eSV/aDVQlVD4wAP86QOo2fVrnnjiCZ566imef/5Njje1O66xnI8+OkdL/zs2tDLSdCpeBSS7s6G2VY96eA4e38iKvBVsPN72zOw8N0Xl1XaOztvLX/oR99//W9pek0p566c/5d1T0FT6Fi+//DJvvfUWe/eWUhdXKSNAuJ/zlMfjAUAHjKo/YruhGdfnD+FYMJake/4WKU0wTYTFgtA0rFYNq03Hqkl0DCxIrBYNqw4WLepQ7Hzu/hN9brXdv7UUdfOM6p5u5rHunnnxvS8gJ9jNxq9/nQceeIBHHnmE73//+zzx5rH+XmCfGPay6rK2iz2fepFV0Zr2+tRZzh1lFZXNwxQCxU8u7SSf6LHz57ebp57t3Dy1M82cOfAWe/fupbK1RGCISD91av+BQnbu2MfWN5vQdQvTpllYuFDj3Z0+Jky8mn/+1jf5l9Wref3159EtpZSdfJstW57jvx//L7733YcYM+Y6Nm1qJiVFJynJit1u45mnSnj1tSKqanf2a2wXRzd6191e7WRYtCavVUaFq7rTtzZ9vPnmm7njjjsoeGZ3pzMeoeiJJ9heBmf2RNd+bxypGpArdNjspKVn4khPJWhEqA/6iZiSMbNmMPOmBaRMnsgHe3fzx++v5frPf5ax47N5+XtraKysIG1KLmOuv5b06dOIRExMKfFHwjg8LsZmZZOSPDA9B7ufp3rXnXZHX9TzkK1rozq1ajNnL2qUfupOH0nY+9Rw16nFS1eSTyHFnd9l44Hkd7XPFr1IGQwETT5aAKcjwdm/DG+dapvfH6aQYtbdFb3/D29um1MuJIuyigvNK10p2l0I+eO62rHGZjGLD6jp8fF2llNHakmdPI4E+395c+t6it7cybYib0J0yuGw8/SGvfz+D3/hw6N/SfBoe+MNnvniF1m9ejU///nPefvt4/gv4Sxz587ltddeQ0rJ7bff3sUJ0d75+/TTTydm6H3ktOHG0DWEBG/AQBPga4lg0+F//yeP3774V3zxKzPJX5zLp79xAys2fIWcuROQgBAaQkhAYgTDBOrtmMEg4bpjJPnLsYZbLvT1feP4DrYX57NwQS6tttdNXW1GPdknels79Pi+245brl8K723g5Xd72KF2P689+yz7uzGfhb3V1Nf7MC/+qhUKhaJftHcCl5ZGq5wMB+cvqB7AlzW61U5zs5+z3nOEZQQTE13o6Gh4hY9TspKfn9vCdE8OU13j0YWOFqvtIuKNxGL/N6RBREY46i1nXPJoLM7+/umUUV6cz8JVl146KTc7H3ZW0E0X37Z9Fiwk/8l1lJ+hNcu3aHchLHuUxSyGZdEM4VP35pIDcL6KD7mSvNGdTnT4/3g++An++s478QDUlXHw7FGqk+Yw2nXJl9AB99kTmFoLNq0Z4WhCt10NJtjsp7GFvDi+cw+aZmLe+h04UgthK/InRYjt/4J49BZY9gwCB4TDoEuECCGJGtadJxoSM8geKWPjJni0pKQ1SrrsuRUsXbWCcT32zylj44ql0dKnJb2U8u4LM5fwyMwlsR7Ai1jWoQdwPQ0tbrIS1K90ZMsJ2FrI9gUvUNJabjbWe2xNds/9nbeuJW9VYYeetBd0GHdLJvO+8m3mxXsAf6ZjD+BAdS1kTSYrQToF0T6luqazo+V9yqmFgIEpog83HQ2SdKYxidmOMXjN0dQGP8GopDcJNlsRRBAYBAM6SbajjPL/kUrPV8E0BqUcdO6EXKBzlYLtrH1sIavb96/qeBQ3LshnXTe9ZqPGinjGQlT/fmZ5iE1vfJmJAHt/xf3r78f+wAa+cFUPg3JNYOJ4H6eqyzjrH0Pm+FRsCbnaGImo2jxwlZ+BzvfxYollAe/cQdm9sKOf2b+7fv0ITZ9bxbe+FTWgndnzPCVFB0i+cy4D1cV+ROnU8Y2s7VyiD1qfaQWPlbB+EcT1obsejNsfW8vCVSWUtMo7+vQr27SCtQtWU1KS2+6cS/lH/ff85J5JvQ7ryNZnCc7/AnfcEe3TVX9qL5VllThnZOHUHVj7eSscjmiKvpBhaNyNGRJYJzVjWzAJfdQoTDOM0CwIKYmY8LtdYSJmO9URRrTnn4BgBOZPtTB7QnR9aLcnIP0/3kP7wRcoubf9/SsE8vt0bPGyRylZ37GHZt7JlV3k15OcVtA2n53982vwwC/5abwrTdW7vLDxL2wZ9XWWzE20ibYjw1pWW9eSt6qMlZtKWtdxRWvWRv8TK5G6fWcZy6e2u+PHN1K4GQoei8qh7LlCeKyEkkVt21fc9TArsqP3P5rtFpVfWae+9/Fjd8WPPfUnfvT/Pcm67EdZ+VfdFdds4qPd2zkTuII5N1/Ralw3AkGw9c8ZPi57Om53CYYRJi3NhpRQUyOx2VJobgpw8uRJamqqSE0Nkptr4vFolJ9p4PSZIH5/M15vhMZGSU6OxpmzAuOYgcUaIcnpIj11AsZgJABfot4tfqSEkkd66OPXrT7u4OcFj/P1um/wo3+5nfYddRoObcF+9T3ceX2Cr60dNrsLm9uD0+OmMhyg2TRITkvlTMl7HNi5E5HsxpKUxLgJE7j6ziVUVpzj0PYdCKFRf6KMiLeFUSmpJE/OwWK1YJcS7DbcKSmkuQfgedDDPHUh3YkSlUth/kpeKIk/+4pYu6aH74rJe8G3LjxPdcTH+dLDNKRMZ8aYmC71s2z5sNepqTeyMH9dl0o88RYgBR3k0vc5aWA4w4GiQ9TPWEBeeuLPPpx1qjVjeuta8jaNi93vItbmFZPfJ/tCTz3oo87kdbHf8jvNTwBMyu4q25xMsnv7uvJqTpHBVelJve11SeROuoqUlN1EIqGE6NSxmE65XR7GZg1SbfoPnuRv7v454//zZdbeOT76Wc1xKupbcDovvkpa3AncORN4KJ2/AHXSgZBgyGhedcSQhPwGqx/I5Ztfi/5VNTW0cODdk8y+biJpozxITUNAzPkrkAg0EcFfA0YAbCmNOIPncIbqCTj73wm8c7XGaG/0nloVdGOf6G3t0Bdufoj/OPAKj6+5k7evv49H/61TWfWMHMaPOcDhyrNcM258uw1eWhqDWEalD2i2229+85sLJizs27cP6FsF02uvvZbrrktg+W6FQjFktHcC33///WzYsGHInb+gHMCXNUK30hL0U9VSDSYIBBKJjk6TaKFRtvDh+YPcIm4hMzm6SOguBydeVhMTypqruDbJj2bpp3XyeNRx20NlnD6ROyEXist7dQB3fXmLL/KjR+Rm58Pmck4BOcDug6/Cld9kVpeV+yw+ceuVtJqP0scwtsFLk6+Z0a7+ZkNHsYYOE7b5sFkCEPEidB9CaFitZ7FOSkafmY38y1vI8lK0SZkQ0TBPlMKLxbB4NqRqyIZmBCYgkTKCpukYaNgaEhT/tvlh8ja3/yCflZvWs3xqLsvXdyyzFO932d753kYCnb8XJI2siYk724iQU6eXVgCWPRp18C5aTcf2R1GHVGFPwRTdOH8HCsfoySSiYFEcKSW6plMVqueV+h1IM4IZs5oLoSE1gUtzcHvyvGipYE2j2v11Upv2ouvNSFNDShNNmIR9GlnBX1Bv/yQh2/hoxuMA9pjtkWJYuKl3Y1I88KVjmabosy9ulI9nsT5UGHP+Alz3VVYc3cmv39nGTVfdQuc4mDjOMdOZkVZHdXUzLfUSXB5stv4tJ3RNI2hE+Kst34kGIsloJmlTsIX1N/8DszOiQR0P7fwFe6qO4rY6u5Yii74PY0qToBEZoPLPPRmK+k5rL+A15RT2t/fvvOV8ZX5b9sSEaddyuqKUuqq5ZA6AB3hE6FTneWrZozEnb5xoSTOWPdrOGJHL8lUr2X7XOgq3Lm/3eTEseKHbIKbiSQVtThSARat54cEyvvzT3/POPQ9xU29jnL6Im6cnt/6aljke76l6gv4snAlI3tH1uD6aEDyLAKQUaJ65sQovsQx5IYgYkv99M4Q/JNHbpY9GzGhP5+aA5L7FNmZPsAMCi6X/rw5FL8SM5d3cv6VP9vHYDgFLi1m9aSVlXeTXs5yWPlnI2/eu5hPA+L/+Dsvbf0nmPObO3clWbwMkPEenI8NZVmVnyiB/YYdgl8WPxNd63ZW07+ocyb13PR1Wh1OXU7BsHQ9X9Lp67/7YnM9x662beabmPHTprtjA0bde53jLTK6/44oOUtMd/c+Ezh57Jenpo3EmaWRmWjBNOHcO3O40vN4Wjh0rxdfixenUmTTJQUqKjcpzXmpqmvE21dLsDeL3w8RJFjKOaAjNJC1dIz09A1dSNk3eRJRp6Gbtl9/mgOqP3vVE9/p4I998rI7a/9jE2wdup6Bd1c3y1FksGeB6jhbdgtORhBRQrxuEAn72vPkm8+Zei3tMFhG7hclNIX5X+DsaTpyipbaG+rPl5H/6dlpCQaqqq6itrqH63T2EgYAmiIRCCJsFhz1B2ZUXnKf6pjtFa2LO3w5OxsWsfqSb72y3nr845y+Aj5AX0rLb6VI/g7aGv051F1DZtQXIxc5JieMIRU+8zDGXC7fbzexF93Brr57HS2ck6FTZmTLyFxRE9eB4BWX54yjow3GtOtTpuVhS0k77YoFjeRWd5dwz56vP0XWegrOVxyEjj4zOwc66A6smMfoRuTBl8rVkZWXjTNLIyopW/uuPTmm6JC1dY/ToTNJSJ2EmpFjPNh65zkmHR9TNa9j/6oPMAF586hH2FPyFZ+5s59AbNZXRzTVcKp2dwDfeeCMvvfTSkDl/ARAapgmakGiaoMlvMHaik3/9h0kYhqSuppkHvvwHzh0o58rJdv7tl3czceZYYsWUoqcgWrUR08QMCnQtCP4KLOHGBAwwXq1xddvc0lPgH/TJPnEp3PRPb3DT9U+x/Ke/4bv3/wnX3Lv517+P2yjSmXXlDA7vOcFHV4xncnx68vpoxs6o/kbT9kJpaSkbNmzoc8W6DRs2XHCfJUuWKAfwMCQvL+/CO/XA5d7zVtE706dP5/777+e73/0u3/3ud4fc+QvKAXzZExaCOsKx30SbE1hq6EIjbEnFrTnRZSzzt8czxbdEnVaJoZuSjBfDhHHkc6GSublkTwJOxpxbxysoI17KpM1Rsu+d1XzipjNUlcOV18/pEK0OwMxxnfpPOLHZgXCECIlRJGErQ9f9CGsIaTSj60GkZsHqqkV3mdG7bg2BuwmcLjCs4PKCJYwQEtPuA2sYNDNmQ5dIrAisQIIMFl1ecDsRj4Ju91H+ma5GvqI1g+X8bYe/gfqAxOVyYbNdes7iiJBTq2O+5z2K1kTLY7WR29UBfGYjKwbJ+dtGiJb6eqQ1atDoD6aMVj14uXE7IWsYWiTESiYhTYTDwg3WmeQ4szGkgQ74HVdS4/8sY9hIqNlC/HlnGjp2ezNjvE9zOuN7IM1ByQLuSh+em3Ej4aaNFCyKO3w79qqKV0L48pSOh44ZNQP21lENPTqAAbCnM3p8erRcrdH/wAUhBKY0eeNsW3kuXWhE/A3UB9tervZUHWX76b3odjdmL2WDPVZntJJFgil7rjB6H/ulD22Ok/72/p1/Radu9alu3ECLvwVIYCp9jBGhU53mqWgJsnbPxFhJs5WrOt35qdFMjrJOc1Y0E78rBTd0lVx035Ocv4Bva0Z2TscPnHaiS4owJKRWRUekiDoVZbtS3RfSjnSXhtUCbnvUOJW41PqOgXjtiVc9uNCxBY91ozU9ZGv1LKcyznWQUwVv/viHvF7hxuVy4XK5cE/z0ghcfM7JpTOcZBUNtFzXY4WQrsFGMYPhg53XdvGgv3Yf5fdewaf9sb954Mv84kh0TeB2u3FdW0MlkNVur1O7XuRA1VxuWjaL7guK9uVO9kyLz0qyx0FOjonLBc0tUFZmMnr0KKw2C+FwhM9/cSk5OZMYPXoCyZ5mgnlH8XiO4fOFsFosjB6VQU1tCIlJ5midyVPB5dJobJIJiifrbe3Xs+5cWO96ohd9nHgNc678A1uOvEfB3La2OuOSU7vum2BMJBqSdIsdi6bTVF/Pe28UUX38I0I+PxZXEmLBQm584D6CDhv+hjRyzAinPjhMoKGBaakZODIyqPf5CSAImdGgsohpJq4H5oXmqVZ6052LCEjr93o+CZsH6stPkzRlIp4ETFMjQae6PONayz+3Zfpe7JyUOGaw+FszWp+1FQde5I3DbqZceyu5Cc4CHs46tSIvr53dYSl57YJZluatA3q2N5Q9t4KHN+ez8kKOq0WrKXkM8lYVsnHp4l7fr+OMGd1NlYr6DzhxAnJumkzi83/BMD2kp7nJyTFxuxOjU7lTJKmpdkJhBwmI/wNuYc3eV3hwVnfbXuStp+BLW27tssWq9++hE3cC33TTTbz00kt85jOfGTrnL4CAiJRYNY0km8Y5v8ln81NI8WggBOt/sp9dW6vJTbVwpPg0m3+whVW/vQ8JyNjDTSKji0bCSOnAYpVgNiJCTb1+dZ+ItzvqUK0xVgZ65w7K7s3tpDP9tOv2xrz72DjvPtj/W7716z/z4283s/z7S5gOkDOWqXv2UF3dzGRP1G7kbakHVw6OATTTTJ8+nbfeeuuC+23YsIGnnnpKOQJHMEp2ikslHijy3e9+lw0bNjBt2rQhdwIrB/BljBkxcFscTHJnQRNII2r8EAiIVXg2MDCRvdpEBAITEzTIdWeRYknCjAxGrbLE0L5cCZ1KmcRfzkrOnoKTJew7BFOWDFD47AWIjGrCEqgHSxgdP8ISBKtAS21G99UiTn4Id3wSNj2HPHEKwjbENdMQn70Bdh5CtFSCOxmhyehaUFjAtKPhJpQ60ObLWAky8lm5qYT1U9s+62JS2vwwDwMFjw2S89d/ntOnGtDcY8gcn9bvcrUjW06x0txPFkeNTyWL233Wec9i1q0qjmaPDJLzt7H8ILVBN2lZE0lz9X960jWdIy2n2R8+CQEdM1baXgiBsGqkmC5uT50X7VuK1mqCqEr+GmlV72KxncEISsBEYGIELKSJN6jxLcGXdIEypQmgu+yrbvtNdUPHMk1tGY/xDIaKk0Dxw8z/ky3q6Igb1l0uXK6c3k/eHt2BVe+/QTTedz7V3ua01IVGnWlg0dre4DxWJ7rdQ6o9qVcHsBnvK5xQJ3DXzI9LJe5UjAcjjRRGok4tfuQFVp5cyroXilj+yGI4U04xxRR3zpSLkd/pt3EDnK02EBhGGF3XAQ3s4yHciF2ayJYPEEIgTTM69wiw6IK/+6SNcHSZ18qW/WGqvdGywylO0ZqhHYn0M6sqVgGmJ8d6n47t3wi6sPuZAtbvcHHNPT9izT9FQwCPvfkEWy+l6dxFMqxltWg1JZvGseKueLZiJ0N6ZwdHvF9cO4NhPNgs/8G21hNFa/J4+GTnL+tK/NgF3/o9b/w0mrFY/OxynqnrtOOhV9jqdjP3U9d0DeAEQr56sPQv+C8cjjA6azRXXDGZsrJKRo02yB4rObC/mvSMTLLHjuVI6RHq6+vRdQumEaHFFwAE6RkZCE2npcVHWpoNiwWCAZ3Zs+cydux4pDQHvqDIQOjOJZwzNXlgM+oBAuEgkVCIDGHFoVsYO2UKwdo6TF0QjIRpOnWGdX9+BM1h5wv/9QOOvr2D9/74J0bNmkna6FHUtrRAOEQgHCZ56mSklJimSTAQJBD0DciYu8xT9EF3+vwsTcR6Pokx06/Ddf4IjZVnibhcuJNTseqX/oc7InSq0zOua/nngZmTLoXsuXdiOfIGJ85VMDY9O6EVlYazTq0vKSEaKLEWVkUDKIrW5FGY3XuwQ/x9uOCxkj45dKOJB4UdK5ud7Eb+p6qoALrLsW8oP0sVU5ne3USVAExTMnbcWKZNm0JZWSUZo/qvU1dffS0TJ+YOTtGrD05xhAsEIPeDn/3sZ4TDYaxWKzt27ODAgQPMnTv3wgcOABZpRN9RBXgcGghwOjRMU6LpsGdnDbpuwWINYXHYqTxRTbDFj93lbA0QjJeDtjg09CQHum6AxULYmtz7l/eBot2FAF2rikQ/7VrZoI/2iX5xzd08cc0stnx7M3987QoeuW06kMPoKXs4WF2Fb7KbJOrxNYA7J/G90BUKhaKvdO75O23aNNUDWDGwRMIRki0OpnsmYBEWNIKdDOKx7IKYoTz+E93SZliXUqIBFmFhmmc8KZYkIuFElCrr3NvyIjlTTjFcuLxPa7mSIthZDJMK2uf2kD0Jnt79Ljts73GI21lyKVUezBBo/XMtBiYl4TlbBtYwmulFCj/C7sCS7EW4BPI3P0L84HnEc88h33gdpI74q1shGIQf/wKRJjEjIbALMCVSWhG6C8wMAlP63wekN7ovQdYDyx7lhexClq7K66YfSC7j8i+1v2x3+Dh/+iy+1HY9q/rJSJZTW5+xvvRhyWflpgLK73q4276Yl2S87426Mk7Vupk4YzKpCWoqGzLCvB3cRkQ7B0iEZsZKC2tghc8l34JHdxExjFj/c4k0TSJ6Gmc8f8fUln/DwIIwwyBktEy3NUiWsZkyYy7oCe1+24luSi9dDO3LNNE54zFWGWHSo+zqYymz3um/VSA+L7V36gokpuyYXWzGPjOl7NUB3P6cCaNL5kfiSbhe2W04ScR8HWVk6lTs7z3OhHHkk8/CC1RJuHQmMWaIrcGBQAibzYEprIiUG7A1H2JXeDSbTjbzrzl1ZDpTMaSJBR2LDssXxu97VKd8QckrB8KAxDAhZ5SIZXoLgsHgUF1Wa5Z2YshlbC6w8394/P9m840nvsPt4y94UMIZ9rKaupz1JcuJZyI+nFfWLksxl+V3FbBuVbREam7nIMuta2PZVZegaxdz7OxPc+eo47y+czO2m5Yxq71x3d9AS9BCsrN/zxYpDTIzM5k+40oOH67E6TDJzpacP3+eGVfOZsyYTPbvL6GutopwyI8QFlpamjGlxOPxABKfz4/HbUMTEAjozJkzj3HjJqFpiaqs1AsJ1Z0BPGcCCAf9NDXWY/oDWEyTZQ89SDgQxN/SQk1DHV5vM5PKF3HwL6/wzJe+itPlYvGX7uKahTdyxi5wZWRgd9hINiJYXU78AT+BcIjmhgbqmzpHHySKTvNUX/7++3z/e1/PXwyeMTOi7ZBCPkJGuF9z9sjQqfZloHOp6BwEOMx0wOVMg4ogQUioA3jY69TxHWwvzqVgKkSDXPNZuLQXyWxd2+r8vdTy3PE2Yl0cwOcq+YBZXNelDUsd5WfPwZRZ3QYqJQJNg+zsbGbNviphOpWXdxM5OVPRB6Po1awcZgzQqdv3/H3ggQe69AQebDI1PzYLGKbEqkmSnTqxwnAAjMp0ARLNoiMNE4vdgm61dqplIhAygiPDjcUZQpgRTM1FwNPfyNV4dYnugihiSR8DVtngQszhhttKOLT9FCdum84UYPyUuZTvqKa2ZTJJIS/1pNHm/+1f9ReFQpE4+lNOeyTR2fkLHXsCD6UTeCD7oiuGmCaflwzNTX76NMJGgIhpxDJ/407eaJaAJjQsmo4QotVgK2Ll5yTRcnKGNAgbQa7PmE665qLJ5+3f4BL1wtSnaLOoU7G4opjy4q7lABffUAD7z3Hg/AG4bTZD9Vjyzp4MnnpEihc9qRbdaERLSUFPaUKMjkDDe8gfFsBHexC3zEPckg8fFCP/+R+h5hgy3UQ4Qog0J1oojCYskJQMZOC9PsG1oPrC1mIKe9iUe+96Xngwn8JVeazd2nV7cUUnF3DM8TIcuOzkFM9q7JbFrC55lILidSxdsbEbx3wsiKMd8YjR4UDE1Dh14hvUHFpHben3qT7y/1Nb+kMqj3wf/+H/4v2jX+CZ/bk8e3AqvzgwhV8cmMKzB6fw9P4cvnf8bzn+5hcJ/ymLxi1X0/yXmXi3zCDyh7loWz0II3FOte6Il0kvuORsjWiZ4eKdOyiKGeXbZxJHDRfFbEvIaD8eFL2wjuL8lW2ZHwNGovXKT6L+XEemTsUy3uNMzSaXYrbv7F+oUeHuoi6fFe0uhLyxXEQO/YDg9UbXaCZgZH6RQv84Vrfcwp9rG3jmg0KEiLYBiZgRTNMkFDHxhyMEw4ApWP9mgFM1JromGJ2sM21sW+nu+Lkvmak3sjC/l/vXK9H1XHfHtgZodFrj9fg9+eN6kdMRzhzocWNCGday6kAuy9c/SgHF0QyoOLFgo+KtRRQ+WUzBXRdyLkUNi5fGLg6/0f2W1Nm3cducTE7te5ljl94qsFcyR2eTd+08vM06DoeD7HFJBAJByspO8v77h5kwMZc5c65m/rwF3HDDPCZPnoorKYk//+nPnDp1lNQ0gdWahDPJRpLLYOaMeYzKGKyqQz3rzqXPL73o4+n9HPwQPjHj6q7bBhgjEqa6poKjZ07RFPSjWa001NfhyUhn+jVzmTVzJp/9m2/yt8//gvlLv8Bdf/93PPJfj3NHwV186trrmSpsTHWnMTN3CqMcSaTbHFhbAtRUnqOxqXaARt1pnuqWzrrTy/3vQsf1fB8S8HvHltSv7N84I0GnchcsJJ9Cip/rbo65+DlpIGnx10NytKVEIhn2OnWmnOJWm1AZ5cW9lKON9cK+WOdvPPs7P97fPv530cmOsevAyzBzLrPHdTrBR+9TbmjNTQAAIABJREFUfH4sc6cMbKTZmKzxzJ/3iYTp1HXXLmbsmMFa1c5g0s3wu11dJ3p/sOWSz9re+fv000+3loOWUnL77bdz4MAgLfjaMYZmkowwUmhIBCluCyfOBEFEbbB3f2U6qal2KioaEJrGJ79xExabJerKFG12WkuShnWUB81igt2GP3k6IUs/kx62FlPYrmVeR6L2BTYX05eZZ8DISaW1nkjKGEZn1FJXV8P5qiqSRqcmrKmaQqFIHGlpA18FaKjpzvkbp70TuLS0dEjGpxzAlzHeZi8iIkjTUljsuY5J9rGEzAhaLApKIBFCwx8OUN1ST4tswmf6QGpIEc0UFlIQMMJMtI1hkeda0kQKIizwNvfXqJTLuPz+GWCLdhfCpL44kqPRu2yO9m7M77zYX5TP5/kjW16C2ZmdV+t9ROu/KjXMmIORGkRL9qJ5DGTjh4js6YhxDnBXI7IiiJb3kD8pQP7HHch/+xzyh3+LaDwCo0ykpRExyYX4f+zde3xU9Z34/9eZW2aSyZVrAuGqAUEJNUFQuXmr0DZWXQkWEdhaC37VglsofFe72l3oD1b6E9u6gltdActK6KpL3IUWRYQgIEkFbJAEISRAuAQyuc8kM+ec7x+TGXKZ3CDJZOD9fDzyIEzmnPmc+ZzPmTPnfd6f95B4tDOXwWJCsdpRY8Mon9CZ9wE3NzRhHBzMYq8/aLGTVYtbv6A0dM5aXp1BkyCwr6+WNHisgPdWN64r3JLYqIGQd44z/kfCCbMCZTX4Z3LUr2368lDuJ9+NFw0vVBRsWNW4tlgz97J08wuMaxoEvmcc6RzktdUNHvtsVZO6wi3oHU0sxRQXl195zBqGnSqcNXUd2KDWhZuNjLLEUXX5Fmou34Tr8ghqSm+m5tLNhFePpKCqF8fLYvm2LJZvy2L4tiyW42VxnHHEcrQikjXuFzEcMeE+asWdG4nnaAT6kXAu6Snolq6o3gTeO2tTWLLl2mtkD71rIuMOvsaSABflh85JJ50M/u9zmyhquNBX/8kHX7e8zprTh8k732Re1GscU6Gh/m7ou1qqteftt5SV1/h1+FrGVSDWSGxWcJZXdEoecCiOKe/NFON44THfaKq/OeL1x5rcgFTAeysD3ejSgkafU75acvDYjFkM6pSWX73y8nJUVcUEqLH38knkTzlfV01/i5WM49v515w/UFlXjclowmAwYDEZsJlNmEwe3v3yNB/nWIi0KlS5YGKSgYFxCugKqqpSXl7e5uu3zps1ypYlLNjQ4N1u134+lNmLX2DcliVNxtpOVs18jYMzXm1+QbeFfkqfOdsbAE7oz638jSNfF/ufc+x/t3Doqrat43pyXxVsWMV7DW9Gqb8o2Hha9PqacJszKGh6jp04gHFNbrbYuXJJizcHNhJg2X3rVrGjlUWiRkzh9kFQnPs5RY76B01GTHjQtGvPCIyIiKNP7yRsVsjLc/NfW6rRdZ3Lly+Ql/c1bk8Nbo+LOnctrtpqFKMbmx2OHz/GhfMlOJ06m9+vpqDATfwAI1ZrX4zGrjqXaOpaxp13+eaBrpbG417eXryRb+59jvSgzK6pUOa4QHbuYY6dP0f15ct8u2cfzlNFlH75Fc5vC7iw7yDqX3OZNHESNrudL/7yvxz94gsKvzhAVeEZnMdOonydj+2CgzhLGMUnCygqLsDTRVNAN/ucatfYaeV4GPBc5Mr5/I+anvu16RJFhY4G/1dvnDHlu2np9UA3AV7FZ1JnyP1vNu5rfKdL5fG9HDkTy+ChDaZ/dlVQVdUZs3b07DFVcLrgyvn5ZwfJaClBoD74O27hn1rtl50rFzT57PNmDI9b2DD7ezbpMyBj8aorgbCs37Fq2xhmzfkeTSsAnyj6GvoPpH8L1Z5UVyUeLfDfOiImph+DB43ttDEVFzcEq7XrS1R5jWThMwsg4x945aMrV3O49C2Oq9xNmgZ/fYIdBI7QXSRZKrBHRaKpGhYLfJ1fRfbfqlAUhe9+fwi/efdB0n+Sys/+7THum3cnqqqiqRq66pviXiNqQBjmqCjMpssQEUFZ/J2opmsLf763OaN5CaoGWrr5oYVnd+BGpcZ2vXonv8tq8uDXH/DbP5/k9pQ7uJI2EUXfPr0oKz1LeWUkdnvDW2Ak+1eInmLVqlXBbkKXai346xPsILBMAX0dq6quIiLcToQezpTI71CpOcmvPYORMG/l3/rs38q6GoorSzjnPkeZWg6KwTtVNGA2mHBrHpJsg3gwegLhmpUqd2UnZBXUT6n0xV4K5gz1n6T765P6vcZjKfWVJ2a8So5/ulLvxfj01e0Ljwy9ayLjXj/Y4O7Qxm2J/w6Qn8ztY6/2rsxrH0rOvjfhGHUnvUv+F00zQsF/odX+A8rfvwdbn0CvcYBuBU8YOM+BywyJ4ejOWqjUUaIHwrLFaKU18PlX6P3sKHXRlN5nxTWsa+ftGTpnLa8Wp7DEXycknVdzXoVANYAbuHdZDq+SwpLFKWTU969/XYtT6i90jOOFzX/ihdWP8UYb7Yi7/TZGHtnDvi2niYgYxR3fG03vwaPRiwq5fMbtrW9qj8JivvptDeV+8l38KfDX8/NO7/OnhasC1ABu4KbZrN0MC2a+xmMpWfVT0vnW1WSMroaUxW2FUG5m1Pg8/verj/koP4LE7zxAyuAEhg3WOXn5DBdqI/x1aa+Fpuk8fMsFdp8xcN4ZjklzUuvR6B1hJjLcjdtZh0FRvLMh+Ke9V6hFJ7ZWY1fiKL4YPYs7i96g0jYWu15FaUxvKqZPQdc0lE648YMtV/rCJ311DjmdccGovnbZwYMBbnzhXpbm/InE52bx4/vfuVID+Pa5/PM/tL7ayjO5HCtrWDM4AoulO+YGC56CDd4biF7t8nrYLY+rKa+cv4r1WYlJ6EfFhSpcVYYbc0yNe4E/5TS9AWItOYmrSPF/znilr85p9+wk6av/xIDNKaQs9r8QL2xuZy25LuZyuXA4HPTu3RubEsbisbM54fiWi04HEeZwNnzzEQcvfM3dCbczMnYYCgYKK8+wtziHI+cuExM9G6fjLhJiPfzoTgsG3QMGE6WOS7hcrmtv4D1L6z8rHiPF99nT3s+Pm2azNieBVSmN+3ncwj+RE2B8ttlPQx7lnxY7+Ps3X+S5//F+7kyZt5K0sO6pAdyz+6qgSf23wFPS3vvYC4yb+RoHZ6SztuEfbprN2tVnG/Vz+ur68762UhAbLHvnm95a9enLP2FhWIAawA30v/V7mI5/zqkT2dQM9Jb/iIy49ulqAazWGEwmG5GRCvn5bv6a48JoVKisLOHEyQoGDO5HTW05NU4rde46NL2KsHA3hae+xV2noRgV/ufjSgYOspA81gpEoWlGFKWzpqs9GLBenz/T7VrGnX+67/px5/tO1mQ8+s4LJix4i/94KAhzqgMYTbhqqig4/Q17D/bj4bsmMjQxka+27yDcbEHTNKxhVuzRUdRUVxNhs3HswEEMJhNmaxgmSxjVlZUoqgrDE/HU1pB79DAXL50GYyddOmnrc6q9Y6eF42H66pzAr1t/Pv/8k7/jx/d/xfNvv8oPmyb3lXzJR5u/pMxuJ+LIn/lf9yS+d6sJSk5wzNng3C8ymms98+v5Ywr81ywOHgx8E2AHP5Pa0vRaiN3+b8z/3nrv+562lP940jtX2alP3uT/33fl+1JExK3c9XAyDWcedtU5IawTAuI9fEwNnbP2ymfPPUtb+A5V4A1sAQdfb3AM9GlUyqr5sTRQxrD/OkZKBhaLhYiIiTz/9qt8r+mhr+RLvv4bjEwbTWvVWTVPHcZrnGs5MrIPRqOVqCjDNY+p79xuIzy8P4pyDRdOmtnFslQby5o8uuB9J6/9EPjhaxyx/5q/+7eFLN13E0lJSdw8+YdMHtjxNrQU/PXxBYGDMR20SdFJtjnYj5UaxYDV7J0C+v8uP876NbcwcEA4935/OPd+f7h/Gd++EWa3ousGwu1Goof1wmBUibS5cEcM53Lf8dfctqyDMG5hSzc8c6U2+ub3SL9ndhtra+HcoZ3++5X7+dTe8JrDWP5u6b9wd7/Gz7MPG0zC53mU9x/BkM6eAkEI0UhHj5Njxozpopb0HO0J/voEczpoJScnp8sLD61bt4633nqLnJwrX0ZSUlL46U9/yvz58zu8vmtZtitMeb8TLoYBnz/e+dl/8f370z8hnldOvM0nZQf5ouwwFlMYiqJgVkzouo5RMXingVaMeHQVt+bBYDDgUutQgAGxUSzu+yTPxKZTVueg7FIZDoejzddu07fvsWBm1tXV4ftsFSmLueYMua62b98+Tp48yRNPPNGu51tL80ja/wwmSyW6rkDy38E9b6CY4tAd+eiuMhRVA90AmgE0BR0zijUGJWEwemU5vPo2bP0WRbsFtxLP8VcG4xoq93q0RvopdHSkr1RVx2hU+OxUOK9lJ6K4q9A0uCkmApNRQdd9dWIb1IfRdTQFwnSdywYzQ1yX+Le9U1GUGCIMFgofm01Nynh0VUXploJIoakj/XTfoVf9PdDwhMSoGCh1lvPFo6u5s7+3KtS0/3mFv5z+ipiwiFZrADfs1U/HLrmGLbn+yZgKDR39nAoLCyMpKQmz2QS6whcXDvHLfWsorrpITFgkNR7vuavZ4P3c8egqmqYRbjFRUVdDv+q/Z/mUmdw9QgWMeDwe8vPzOycA3JIQObdryw3RVw1dy/l8EHW0n3Qd3G4X//TKfXx7vI7TRWC16SgKmMwG4gdFYLfbsFqtaJpKaamLy5dqKD3vxOPRMRgVFCA1dQj3P3Ar3//eMiyWMCRDpW0d6auUVc94P4I8dSQOuYVbEm9hXEQc9946hjpnLX369UMzGTn81VdMmnAn1qhIPt27B5PRTC+LhZioKMyKgQMFx9l96Rwnyy/wzd/246xzgtFMztJ/6/oNDlEypnqSOqodDkyR/QgL8PVSxlTPoeu6/0dVVVRVpa6ujurqahISEhg1alQH1gVOZxU/emIQx/Nrr3pM3XHHMKZ/7zs89+w6rFYboTimnn76aYCAwd+GDh8+THp6OhkZGZ0SAN62bRt2ux2bzYbRaMRoNGIwGLyzK3pTd9E0b5m9bef7suN4HI68s5QXVFF5qpzUYbB00TBGjIgiPMJEdVk154+fIzzcTK+EGJyl5Xyx8HUGmIvoPywGm3qZQUlFFI5fyOXe46n/MnbN2xFaznP88zzCxkxhUBuzzB45coSvv/663Z9T1yJQHESIUPf555/z7rvvcuTIkTafm5yczNy5c5kyZUo3tCx4fvCDH3Q4mOsLGn/88cdd2LLGuiXakJDgrcVSXFzs//1q+dKkk5KSrrld1zuz2YzJZEJBIavsMJV6NcOjBxBv6EtxXQnFrksYDUbcuoqiq7jwTr1qxECdp46hln6MCB/MtL7juDP8NlTVTbmjnJqaTpr+6qbZpM94jSV/2snsDtwJ5ruDc9zCP4X0BcJmdB1X3AhOj3meocXLUcIM6Of+C/3Ph9CHPIoh6mYw2EAx4D0J9wYXFTcoZR60gx/Czi9RvqmC2CHolW7Ozu3lDSrekCeCXUT6KWQYjQqaBvcMqeGzwgoOlsQyyFSN1aSg6jpGg+Kvie4PPSpg1HXcRoX+qouD4QP4y82/5Iljv6B49A+pSRkPmiaBqk7k6wGNxpcX9PoAr6prqJrW6DG9leBvw3V1+R1uNxgZU6GjtraWM2fOMHToUDQ07uo3ljem/BO/O/weu4u/REfBbDCh6d6x5Rt7FbV1pPZN5rn7b+KOfiq6bgR0Tp8+3eUBRW9t3hfanYV9vQjFvmrIVx99aQgFf6+eQnLyOKoqT1J8tpjUcVbCw3VsNoWSUgNRUW6iotx4PCq9YnQGxitc7GUhzAoRESZ2flpNnz4DGDPmLryVmOScr9Pp9TcgmcycLjqGy1mNetsEEk3w0B3jiI/0TmeaPHIUlaqby65qUh/8Lio6sRYrMR6NI2eLOHxG5+vzJzn17WFq3XVgNIPeCXO0iiZkTHWJuhrqsGPrjKt9MqZCisFgJDX1Lqqr8ik+e+aqxlR8/GDGjbsHRTESqmOqrcCvT3JycrdPxWkweN/T7/a7xIUSjUOxsVScqiTCZuTbbyv42fw93DbMwMDeOuWnzlL+txP0s6tEGWuJUDzcNhJ6DbSjuuqIG2zhwq2P38DBX6g6WUgxCYy5/kuMChF0U6ZMue4Duh11NUHcESNGdGvwF7opADx16lTsdjuLFy/m5ZdfvuoU57y8PH71q19ht9tJTU3t5FZef2zhNowG7wXVI9XHiY+MY2zUcEbqN/HX6mOU1VVgNJpQ0eqv0yreC7UY8FDHreFD+W7UeObHPIpHdVNRW0nZ5TL/BajOcO+yV0lPWUIK7Z0OpID3FjzGa7zAn7p8Ks5uVp815Rj4CBZLGQOqf49iUdC1ApRv/xVN0VAMCqig1+pQi3d64QoLusMEl2zgHojSqz+U1XL6+wMpnRRzw54IdhnppxDjvWjx5K2XOHcwklhrLLquYFAUlFbechPg1mGYAm/e9Dj3OvdTft89qCoYlFD9Ktwz+QK1BgBF8Qd3FUUBXSfSbMNYPzWwyWD0liho8Lx

Keywords