Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex

Julia A. M. Bakx; Andreas S. Biebricher; Graeme A. King; Panagiotis Christodoulis; Kata Sarlós; Anna H. Bizard; Ian D. Hickson; Gijs J. L. Wuite; Erwin J. G. Peterman

doi:10.1038/s41467-022-28082-5

Nature Communications (Jan 2022)

Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex

Julia A. M. Bakx,
Andreas S. Biebricher,
Graeme A. King,
Panagiotis Christodoulis,
Kata Sarlós,
Anna H. Bizard,
Ian D. Hickson,
Gijs J. L. Wuite,
Erwin J. G. Peterman

Affiliations

Julia A. M. Bakx: Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam
Andreas S. Biebricher: Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam
Graeme A. King: Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam
Panagiotis Christodoulis: Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam
Kata Sarlós: Center for Chromosome Stability and Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen
Anna H. Bizard: Center for Chromosome Stability and Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen
Ian D. Hickson: Center for Chromosome Stability and Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen
Gijs J. L. Wuite: Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam
Erwin J. G. Peterman: Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam

DOI: https://doi.org/10.1038/s41467-022-28082-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Here the authors probe the cleavage and gate opening of single-stranded DNA by the human topoisomerase TRR using a unique single-molecule strategy to reveal structural plasticity in response to both double-stranded DNA and the helicase BLM.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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