Nature Communications (Dec 2022)

Compact Cas9d and HEARO enzymes for genome editing discovered from uncultivated microbes

  • Daniela S. Aliaga Goltsman,
  • Lisa M. Alexander,
  • Jyun-Liang Lin,
  • Rodrigo Fregoso Ocampo,
  • Benjamin Freeman,
  • Rebecca C. Lamothe,
  • Andres Perez Rivas,
  • Morayma M. Temoche-Diaz,
  • Shailaja Chadha,
  • Natalie Nordenfelt,
  • Owen P. Janson,
  • Ian Barr,
  • Audra E. Devoto,
  • Gregory J. Cost,
  • Cristina N. Butterfield,
  • Brian C. Thomas,
  • Christopher T. Brown

DOI
https://doi.org/10.1038/s41467-022-35257-7
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

Programmable, RNA-guided nucleases are diverse enzymes that have been repurposed for biotechnological applications. Here, the authors mine an extensive genome-resolved metagenomics database and identified uncharacterized families of RNA-guided, compact nucleases.