Generation of IPi002-A/B/C human induced pluripotent stem cell lines from MARCH amniotic fluid cells

Abstract

Human induced pluripotent stem cells (hiPSCs) have become a revolutionary tool in biomedical research due to their unique in vitro properties and fate versatility. They offer insights into development or genetic disorders, facilitate drug discovery and hold promise for regenerative medicine. Here we generated three hiPSC cells – IPi002-A/B/C – from primary amniotic fluid cells (AFCs) obtained via amniocentesis for the prenatal diagnosis of MARCH syndrome: Multinucleated neurons, Anhydramnios, Renal dysplasia, Cerebellar hypoplasia, and Hydranencephaly. These AFCs underwent reprogramming through non-integrative viral transduction and the resulting hiPSCs exhibited normal karyotype and expressed typical pluripotency markers.