The Histopathological and macroscopical considerations of induced experimental periodontitis in rats

Abstract

Ten Wistar males rats were used to induce experimental periodontitis by placing a 5-0 cotton thread ligature at the base of the first superior molar on the left side. Before this phase, the molar went through the process of scaling, rooting and planning. Soft movements of the molar were realized for creating an accumulation of plaque by flattening and by resulting the displacement of the gingival tissue, thus provoking an inflammatory response. After 7 days the ligatures were removed in all ten rats. After 14 days, results obtained showed gross aspects of periodontitis and microscopical lesions as well, installed in the periodontium. Microscopically, an inflammatory response with bone necrosis and alveolar bone loss was observed. The aim of this study is to test an experimental protocol of periodontitis confirming the presence of this pathology by gross aspects and also histopathological aspects. In conclusion, the tested procedure is able to provide all the key biological elements present in periodontal disease, whereas representing the adequate features for biomaterial testing domain.

Keywords

• chronic inflamation
• bone necrosis
• alveolar bone loss
• rats
• experimental periodontitis