Amplification of Femtograms of Bacterial DNA Within 3 h Using a Digital Microfluidics Platform for MinION Sequencing

• Yuguang Liu,
• Patricio Jeraldo,
• Helena Mendes-Soares,
• Thao Masters,
• Abigail E. Asangba,
• Heidi Nelson,
• Robin Patel,
• Nicholas Chia,
• Marina Walther-Antonio

Affiliations

Yuguang Liu

Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota, United States

Patricio Jeraldo

Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota, United States

Helena Mendes-Soares

Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota, United States

Thao Masters

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, United States

Abigail E. Asangba

Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota, United States

Heidi Nelson

Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota, United States

Robin Patel

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, United States

Nicholas Chia

Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota, United States

Marina Walther-Antonio

Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota, United States