APPLICATION OF NANOPARTICLES BASED ON RARE EARTH ORTHOVANADATES TO INACTIVATE EHRLICH CARCINOMA GROWTH

Abstract

The research of the peculiarities of Ehrlich carcinoma growth in vivo after incubation with nanoparticles based on rare-earth orthovanadates of spherical, spindle-like and rod-like shapes under different concentrations was the aim of this study. By immune fluorescence method there were quantitatively assessed the tumor precursors of various differentiation rate on the presence of phenotype markers CD44, CD24, CD 117 and Sca-1. The inhibition of tumor process after pre-treatment of Ehrlich carcinoma cells with nanoparticles of all the shapes and concentrations has been demonstrated. Nano-spindles of 0.875g/l concentration were in a greater extent capable of tumor growth inhibiting that stipulates a maximal survival of tumor-bearing mice. There has been shown a significant re-distribution in growing tumor of the content of the precursors with the mentioned above phenotype markers after pre-treatment of inoculated Ehrlich carcinoma cells with nanoparticles of all the shapes and concentrations. Predictive value of the coefficient of CD44hi to CD117+ cells’ ratio when assessing the anti-tumor therapy was found.

Keywords

• nanoparticles
• cancer stem cells
• Ehrlich carcinoma.